Method and device for detecting mutated proteins

Abstract

The invention provides a method and a device for detecting mutated proteins. The method includes acquiring transcriptome data corresponding to samples; comparing the transcriptome data to mitochondrion databases and outputting non-mitochondrion sequences according to comparison results of the transcriptome data and the mitochondrion databases; transforming nucleotide sequences in the non-mitochondrion sequences into amino acid sequences, comparing the transformed amino acid sequences to protein databases and extracting amino acid sequences with homogenous rates in first set ranges and amino acid lengths in second set ranges from comparison results of the transformed amino acid sequences and the protein databases; comparing the extracted amino acid sequences with the homogenous rates in the first set ranges and the amino acid lengths in the second set ranges to NCBI (national center of biotechnology information) and determining the mutated proteins according to comparison results of the extracted amino acid sequences and the NCBI. According to the scheme, the method and the device have the advantage that the mutated proteins in the samples can be detected by the aid of the method and the device.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method and device for detecting a mutant protein. Background technique [0002] Cancer refers to malignant tumors originating from epithelial tissue. Common malignant tumors include liver cancer and lung cancer. Worldwide, the number of deaths caused by malignant tumors accounts for one-eighth of the total number of deaths, more than the total number of deaths from AIDS, tuberculosis and malaria, and the incidence of malignant tumors is increasing year by year. The occurrence of malignant tumors is the result of the joint action of the environment and genes, and gene mutations can lead to protein mutations. Therefore, the study of mutant proteins is still a research hotspot. [0003] In the prior art, the analysis of protein mutation is generally carried out by establishing a series of electrophoresis and analyzing the DNA conformation in the sample, while the prior art only analy...

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Problems solved by technology

[0003] In the prior art, protein mutation analysis is generally performed by establishing a series of electrophoresis and analyzing the DNA conformation in the sample, while the prior art only analyzes whether a certain protein has a mutation, and cannot detect the mutation of the sample. What are the mutated proteins in

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