Nano-gold self-assembled Si sheet material and application thereof

A nano-gold, self-assembly technology, applied in the direction of nanotechnology, analytical materials, material excitation analysis, etc., to achieve good uniformity and stability, simple and fast method, and good synergistic effects

Inactive Publication Date: 2017-12-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no SiO 2 Self-assembled gold nanoparticles coated on the surface of Si sheet for SERS imaging of suspended cells

Method used

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  • Nano-gold self-assembled Si sheet material and application thereof
  • Nano-gold self-assembled Si sheet material and application thereof
  • Nano-gold self-assembled Si sheet material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 nano-gold self-assembled Si sheet material

[0028] (1) SiO 2 @Si chip cleaning

[0029] SiO 2 @Si slice (i.e. SiO 2 Coated Si wafers (purchased from Zhejiang Lijing Silicon Materials Co., Ltd.) were immersed in acetone, ethanol and deionized water in turn, cleaned by ultrasonic waves at 100W for 15min, and then dried with nitrogen to obtain SiO after cleaning. 2 @Si slice.

[0030] (2) activation

[0031] SiO after step (1) cleaning 2 @Si sheet undergoes activation treatment to form SiO with amino terminal on the surface 2 @Si slice:

[0032] SiO after step (1) cleaning 2 The @Si piece is immersed in 1mol / L NaOH aqueous solution, stirred ultrasonically at 100W for 10min, so that the surface is covered with a layer of hydroxyl group (-OH), take it out and rinse it with deionized water, dry it at room temperature, and then put it in a concentration of 1% Soak in a methanol solution of 3-aminopropyl-trimethoxysilane (APTMS) for 24 hours at room tempe...

Embodiment 2

[0038] (1) SiO 2 @Si chip cleaning

[0039] With embodiment 1.

[0040] (2) activation

[0041] SiO after step (1) cleaning 2 @Si sheet undergoes activation treatment to form SiO with amino terminal on the surface 2 @Si slice:

[0042] SiO after step (1) cleaning 2 The @Si piece is immersed in 2mol / L NaOH aqueous solution, stirred ultrasonically for 20min at 100W, so that the surface is covered with a layer of hydroxyl group (-OH), taken out, rinsed with deionized water, dried at room temperature, and then put into a solution with a mass concentration of 2%. Soak in methanol solution of 3-aminopropyl-trimethoxysilane (APTMS) at room temperature for 12 hours to silanize the surface of the substrate and expose the amino group at the end, take it out and rinse it with deionized water, dry it at room temperature, and then put it in 0.2mol / L Stand in the aqueous HCl solution for 40 minutes to protonate the terminal amino group by electrostatic adsorption, take it out, wash it...

Embodiment 3

[0048] (1) SiO 2 @Si chip cleaning

[0049] With embodiment 1.

[0050] (2) activation

[0051] SiO after step (1) cleaning 2 @Si sheet undergoes activation treatment to form SiO with amino terminal on the surface 2 @Si slice:

[0052] SiO after step (1) cleaning 2 The @Si piece is immersed in a 1mol / L NaOH aqueous solution, stirred ultrasonically at 100W for 10min, so that the surface is covered with a layer of hydroxyl (-OH), taken out, rinsed with deionized water, dried at room temperature, and then put into a solution with a mass concentration of 3%. Soak in methanol solution of 3-aminopropyl-trimethoxysilane (APTMS) for 18 hours at room temperature to silanize the surface of the substrate and expose the amino group at the end, take it out and rinse it with deionized water, dry it at room temperature, and then put in 0.3mol / L Stand in HCl aqueous solution for 60min to protonate the terminal amino group by electrostatic adsorption, take it out, wash it with deionized ...

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Abstract

The invention discloses a nano-gold self-assembled Si sheet material and application of the nano-gold self-assembled Si sheet material. According to the material, the SiO2-coated Si sheet serves as a substrate, the surface of SiO2 is subjected to amination modification, and the amination-modified SiO2-coated Si sheet is obtained; and then the surface of the amination-modified SiO2-coated Si sheet is formed by self-assembling nano-gold particles. According to the nano-gold self-assembled Si sheet material and application, the SERS substrate is produced by the adoption of the self-assembly manner, and the method is simple and convenient and fast to implement; and the enhancement effect is better when the prepared nano-gold self-assembled Si sheet material is used in an SERS imaging study of suspension cells, imaging of the cells suspension-cultured by the same culturing method can be achieved by means of the method, and adherent-cultured cells can be injected to the substrate dropwise after trypsinization by means of the same method for the test.

Description

(1) Technical field [0001] The invention relates to a nano-gold self-assembled Si sheet substrate, and the surface-enhanced Raman spectrum active substrate is used in a method for suspended cell Raman imaging. (2) Background technology [0002] Surface Enhanced Raman Scattering (SERS) is a special surface optical phenomenon discovered by researchers using laser Raman spectroscopy technology for surface science research. It can amplify the Raman signal adsorbed on the surface of the material by 10 6 times, up to 10 14 . The discovery of SERS has given Raman spectroscopy great potential in the application of receptors and single-molecule detection. [0003] The surface morphology of the substrate adsorbed by the detected substance molecules is an important factor for the occurrence of SERS and the strength of the Raman signal. Therefore, the study of SERS active substrates has always been one of the research hotspots in this field, and it is important to expand the applicati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C23C18/04C23C18/08G01N21/65B82Y40/00
CPCC23C18/04B82Y40/00C23C18/08G01N21/658
Inventor 徐宁董莹莹徐楠楠
Owner ZHEJIANG UNIV OF TECH
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