The invention relates to a two-dimensional flexible device for Raman quantitation and
imaging analysis and a preparation method thereof. The device is composed of two single-layer
graphene sheets (1LG) prepared by
chemical vapor deposition and single-layer self-assembled gold nanostars (AuNSs) clamped between the two
graphene sheets, and is fixed by a
polymethyl methacrylate (PMMA) membrane. The method comprises the following steps: fixing an
internal standard (IS) on the upper 1LG surface of the device, and detecting a to-be-detected substance by using the lower 1LG surface of the device, thereby simultaneously acquiring non-interfering IS and a surface enhanced
Raman scattering (SERS)
signal of the to-be-detected substance under excitation of light of certain
wavelength, and further obtaining reliable SERS quantitative results by using the IS method. The device has an ultra-thin two-dimensional structure and excellent flexible characteristics and can be attached to the surface of any object to realize detection of multiple samples on solution and
solid surfaces, and the SERS imaging result of the to-be-detected substance on the
solid surface can be obtained based on the SERS
signal. The device has excellent stability,
reusability and structural variability, can be used for Raman quantitative detection and
imaging study of multiple to-be-detected substances on multiple occasions, and has wide application prospects.