Artificial liposome containing miRNA mimic and preparation method and application thereof

A technology of liposomes and artificial lipids, which is applied in biochemical equipment and methods, microbiological determination/testing, etc. It can solve the problems that are difficult to normalize with markers, interference factors cannot be ruled out, and clinical sample capsules cannot be effectively used. Bubble miRNA abundance analysis and other issues
CN110724734AActive Publication Date: 2020-01-24北京恩泽康泰生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
北京恩泽康泰生物科技有限公司
Publication Date
2020-01-24

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Abstract

The invention provides an artificial liposome containing miRNA mimic and a preparation method and application thereof. The artificial liposome is prepared by mixing liposome with a lipid bilayer structure and miRNA mimic, wherein the miRNA mimic is a synthetic non-human miRNA and its derivatives. A synthetic liposome wrapped with the exogenous miRNA mimic is used for characterization of miRNA extraction efficiency of extracellular vesicle samples and quantitative analysis of extracellular vesicle miRNA in body fluid samples, thus providing strong technical support for stable quantitative detection of vesicle miRNA in clinical needs.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to an artificial liposome containing miRNA mimic and its preparation method and application. Background technique

[0002] Extracellular Vesicles (EVs) refer to vesicle-like bodies with a double-membrane structure that are shed from the cell membrane or secreted by cells, with diameters ranging from 30-1000 nm. Extracellular vesicles are mainly composed of microvesicles. (MicroVesicles, MVs) and exosomes (exosomes), microvesicles are small vesicles that are shed from the cell membrane after cell activation or injury. Due to the unique biological characteristics of extracellular vesicles, they are of great significance in disease diagnosis, especially exosomes.

[0003] Exosomes are a kind of membranous vesicles with a particle size of 30-150 nm secreted into the extracellular environment after the fusion of intracellular multivesicular bodies and cell membranes. It plays an important ...

Claims

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