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Method for determining bacterial amount in sodium alginate microcapsule

A technology of sodium alginate and determination method, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, color/spectral property measurement, etc., can solve the problem of high cost of qPCR method, long determination time of glucosamine method, ergosterol It can solve problems such as the measurement of discomfort in the method, and achieve the effect of simple and easy operation.

Active Publication Date: 2019-04-16
FUZHOU UNIVERSITY
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Problems solved by technology

For the rapid determination of bacteria and fungi, there are deficiencies, such as: the ergosterol method is not suitable for the determination of bacterial count; the glucosamine method takes a long time to measure; the dry weight method is only suitable for liquid free fermentation; high

Method used

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  • Method for determining bacterial amount in sodium alginate microcapsule
  • Method for determining bacterial amount in sodium alginate microcapsule
  • Method for determining bacterial amount in sodium alginate microcapsule

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Determination of the amount of bacteria in the microcapsules of Monascus

[0026] (1) Configuration of various reagents:

[0027] ① Anaerobic sodium citrate solution: accurately weigh 0.2moL sodium citrate, dilute the volume to 1L with deionized water after boiling and cooling, and remove oxygen again by 100W ultrasound for 20min.

[0028] ②27wt% anaerobic sulfuric acid solution: slowly add 98wt% concentrated sulfuric acid to the deionized water after boiling and cooling to dilute the concentrated sulfuric acid concentration to 27wt%, and then remove oxygen again by 100W ultrasound for 20min.

[0029] ③Standard phosphorus solution: Bake potassium dihydrogen phosphate at 110°C to constant weight, accurately weigh 0.8775g, dissolve in a small amount of distilled water, transfer to a 500ml volumetric flask, and dilute it 20 times before use.

[0030] (2) Dissolution of sodium alginate microcapsules: place the sodium alginate microcapsules in a 75-fold volume of 0.2mol / L an...

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Abstract

The invention provides a method for determining the bacterial amount in a sodium alginate microcapsule. For the sodium alginate microcapsule after immobilized fermentation, an anaerobic sodium citratesolution is used to dissolve the microcapsule under a heating condition. The dissolved solution is taken to determine the content of organic phosphorus. The amount of organic phosphorus in the capsule is used to characterize the bacterial amount in the capsule. Since the phosphorus content of bacteria is relatively stable, the amount of bacteria can be characterized by the amount of organic phosphorus. In addition, the sodium alginate envelope dissolution method provided by the invention can avoid the conversion of organic phosphorus to inorganic phosphorus when an envelope is dissolved, so that the determination of the content of total phosphorus and inorganic phosphorus is not affected. The entire determining process has the advantages of convenient operation, being fast and accurate and easy implementing.

Description

Technical field [0001] The invention belongs to the technical field of measuring the amount of bacteria, and specifically relates to a method for measuring the amount of bacteria in a sodium alginate microcapsule. Background technique [0002] There are many methods to determine the amount of bacteria, such as: glucosamine method, ergosterol method, dry weight method, qPCR method and so on. For the rapid determination of bacteria and fungi, there are shortcomings, such as: the ergosterol method is not suitable for the determination of bacterial counts; the glucosamine method has a long measurement time; the dry weight method is only suitable for liquid free fermentation; the qPCR method is more costly high. [0003] For sodium alginate-immobilized cell microcapsules, if you want to determine the amount of bacteria in the capsule, you must first dissolve the membrane, and the method of dissolving the capsule may interfere with the measurement of the bacterial count, so it is necess...

Claims

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Application Information

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IPC IPC(8): G01N21/31C12Q1/06
CPCC12Q1/06G01N21/31
Inventor 倪莉周康熙刘志彬张雯张晨
Owner FUZHOU UNIVERSITY
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