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A method for measuring the amount of bacteria in sodium alginate microcapsules

A sodium alginate and determination method technology, applied in biochemical equipment and methods, color/spectral characteristic measurement, microbial determination/inspection, etc., can solve the high cost of qPCR method, unsuitable determination of ergosterol method, and glucosamine method Long measurement time and other problems, to achieve the effect of simple operation

Active Publication Date: 2021-07-13
FUZHOU UNIV
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Problems solved by technology

For the rapid determination of bacteria and fungi, there are deficiencies, such as: the ergosterol method is not suitable for the determination of bacterial count; the glucosamine method takes a long time to measure; the dry weight method is only suitable for liquid free fermentation; high

Method used

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  • A method for measuring the amount of bacteria in sodium alginate microcapsules
  • A method for measuring the amount of bacteria in sodium alginate microcapsules
  • A method for measuring the amount of bacteria in sodium alginate microcapsules

Examples

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Embodiment 1

[0025] Example 1 Determination of the amount of bacteria in the Monascus microcapsule capsule

[0026] (1) Configuration of various reagents:

[0027] ① Anaerobic sodium citrate solution: Accurately weigh 0.2 mol of sodium citrate, dilute to 1 L with boiled and cooled deionized water, and remove oxygen again by ultrasonication at 100 W for 20 minutes.

[0028] ②27wt% anaerobic sulfuric acid solution: Slowly add 98wt% concentrated sulfuric acid to deionized water after boiling and cooling to dilute the concentration of concentrated sulfuric acid to 27wt%, and then 100W ultrasonic for 20min to remove oxygen again.

[0029] ③Standard phosphorus solution: bake potassium dihydrogen phosphate at 110°C to constant weight, accurately weigh 0.8775g, dissolve in a small amount of distilled water, transfer to a 500ml volumetric flask, and dilute 20 times before use.

[0030] (2) Dissolution of sodium alginate microcapsules: place the sodium alginate microcapsules in a 0.2mol / L anaerobic...

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Abstract

The invention provides a method for measuring the amount of bacteria in sodium alginate microcapsules. For the sodium alginate microcapsules after immobilization and fermentation, anaerobic sodium citrate solution is used to dissolve the microcapsules under heating conditions, and the solution is taken The content of organic phosphorus was measured, and the amount of organic phosphorus in the capsule was used to characterize the amount of bacteria in the capsule. Since the phosphorus content of the bacteria is relatively stable, the amount of organic phosphorus can be used to characterize the amount of bacteria. In addition, the sodium alginate capsule dissolution method of the present invention can avoid the conversion of organic phosphorus to inorganic phosphorus when the capsule is dissolved, and does not affect the determination of total phosphorus and inorganic phosphorus content. The whole determination process is convenient, fast, accurate and easy to implement.

Description

technical field [0001] The invention belongs to the technical field of measuring the amount of bacteria, in particular to a method for measuring the amount of bacteria in sodium alginate microcapsules. Background technique [0002] There are many methods for the determination of bacterial counts, such as: glucosamine method, ergosterol method, dry weight method, qPCR method, etc. For the rapid determination of bacteria and fungi, there are deficiencies, such as: the ergosterol method is not suitable for the determination of bacterial count; the glucosamine method takes a long time to measure; the dry weight method is only suitable for liquid free fermentation; high. [0003] For sodium alginate-immobilized cell microcapsules, in order to measure the amount of bacteria in the capsule, the membrane must be dissolved first, and the method of dissolving the capsule may interfere with the determination of the bacteria, so it is necessary to construct a suitable dissolved capsule...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31C12Q1/06
CPCC12Q1/06G01N21/31
Inventor 倪莉周康熙刘志彬张雯张晨
Owner FUZHOU UNIV
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