Preparation method of magnetic and luminous bifunctional hydroxyapatite nanoparticle in core-shell structure

A technology of hydroxyapatite and nano-core shell, which is applied in the direction of nanotechnology, luminescent materials, nanotechnology, etc., can solve the problems of application effect, difficult enrichment and separation of labeled particles, difficult labeling particle destination, etc., and achieve biocompatibility good performance and stable structure

Inactive Publication Date: 2014-05-28
NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Luminescent labeling is favored by people because of its easy detection and high sensitivity. However, in the process of luminescent labeling experiments, the labeling particles can only play a simple marking role. Functional defects of luminescent labeling particles that act on the labeled substance throug

Method used

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  • Preparation method of magnetic and luminous bifunctional hydroxyapatite nanoparticle in core-shell structure
  • Preparation method of magnetic and luminous bifunctional hydroxyapatite nanoparticle in core-shell structure
  • Preparation method of magnetic and luminous bifunctional hydroxyapatite nanoparticle in core-shell structure

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A: Fe 3+ and Co 2+ Doped hydroxyapatite magnetic core (Fe 3+ / Co 2+ -HAP) prepared by hydrothermal method

[0034] (1) Get 0.420g cetyl ammonium bromide (CTAB) and 12mmol diammonium hydrogen phosphate ((NH 4 ) 2 HPO 4 ) was dissolved in 24mL of distilled water, and magnetically stirred until uniformly mixed to obtain solution A.

[0035] (2) Take 4mL1mol / L mixed solution of ferric nitrate and cobalt nitrate (Fe 3+ :Co 2+ =2:1) ​​and 16mmol Ca(NO 3 ) 2 .4H 2 O was placed in 36mL of distilled water, and magnetically stirred until uniformly mixed to obtain solution B.

[0036] (3) Add solution B to solution A at a rate of 2mL / min, adjust its pH to 10 with 2mol / L NaOH solution, and pour the suspension into a polytetrafluoroethylene-based reactor after stirring for 0.5h , reacted at 140°C for 8h.

[0037] (4) The resulting precipitate is filtered, washed twice with distilled water and absolute ethanol, and the magnetic core of hydroxyapatite (Fe 3+ / Co 2+ -HAP...

Embodiment 2

[0049] A: Fe 3+ and Co 2+ Doped hydroxyapatite magnetic core (Fe 3+ / Co 2+ -HAP) prepared by hydrothermal method

[0050](1) Get 0.420g cetyl ammonium bromide (CTAB) and 12mmol diammonium hydrogen phosphate ((NH 4 ) 2 HPO 4 ) was dissolved in 24mL of distilled water, and magnetically stirred until uniformly mixed to obtain solution A.

[0051] (2) Take 4mL1mol / L mixed solution of ferric nitrate and cobalt nitrate (Fe 3+ :Co 2+ =2:1) ​​and 16mmol Ca(NO 3 ) 2 4H 2 O was placed in 36mL of distilled water, and magnetically stirred until uniformly mixed to obtain solution B.

[0052] (3) Add solution B to solution A at a rate of 2mL / min, adjust its pH to 10 with 2mol / L NaOH solution, and pour the suspension into polytetrafluoroethylene as the substrate after stirring for 0.5h Reactor at 140°C for 8h.

[0053] (4) The resulting precipitate is filtered, washed twice with distilled water and absolute ethanol to obtain the magnetic core of hydroxyapatite (Fe 3+ / Co 2+ -H...

Embodiment 3

[0065] With reference to the preparation steps of Example 1, the difference is that Tb in step (10) 3+ accounted for (Ca 2+ +Tb 3+ ) The mole fraction is 8%. Depend on Figure 7 It can be seen that compared with Example 1, Example 3 increases Tb 3+ The fluorescence intensity at 544nm was significantly enhanced with the doping amount.

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Abstract

The invention discloses a magnetic and luminous bifunctional hydroxyapatite nanoparticle in a core-shell structure and belongs to the technical field of nano functional material preparation. According to the method, pure hydroxyapatite continues to grow on the surface of a magnetic hydroxyapatite nanoparticle by controlling a coprecipitation condition to realize hydroxyapatite thin layer coating of the magnetic particle, so that dissolving of magnetic ions (such as Fe<3+>) quenching luminescence of a rare earth ion is inhibited. Rare earth doped hydroxyapatite continues to grow on the surface of the magnetic hydroxyapatite nanoparticle with a hydroxyapatite coating layer, so that the magnetic and luminous hydroxyapatite nanoparticle in the core-shell structure is obtained. The core-shell type magnetic and luminous hydroxyapatite nanoparticle has good magnetic property, luminescence property and biocompatibility, further has target positioning and mark tracking functions, and has good application prospects in the field of biomedicine such as substance detection inside and outside a cell, nucleic acid hybridization and sequencing, early disease diagnosis, transport of gene drugs, and magnetic heat treatment.

Description

technical field [0001] The invention belongs to the technical field of preparation of nano functional materials, in particular to a method for preparing magnetic and luminous double-functional nano core-shell structure hydroxyapatite particles. Background technique [0002] Luminescent labeling has played an increasingly important role in biological and medical research fields such as the detection of intracellular and extracellular substances, hybridization and sequencing of nucleic acids, early diagnosis and treatment of diseases, and drug development. The most commonly used markers for luminescent labeling methods are organic fluorescent markers. Organic fluorescent markers have some insurmountable defects, such as unstable fluorescent signals and toxic side effects in most fluorescent reagents. Therefore, the research and development of new and highly sensitive inorganic nanoluminescent markers has attracted much attention. Rare earth-doped inorganic nanocrystal lumine...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/71C09K11/81C01B25/32B82Y30/00B82Y40/00H01F1/11H01F1/36
Inventor 刘志刚王俏杨立荣姚少威于守武白瑞英封孝信
Owner NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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