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Hydroxyapatite nanoparticle with antitumor activity, preparation method and application thereof

A technology of hydroxyapatite and nanoparticles, which can be used in the fields of antineoplastic drugs, active ingredients of phosphorus compounds, powder delivery, etc. It can solve problems such as different preparation methods, no antitumor effect of nanoparticles, and uneven antitumor effects.

Inactive Publication Date: 2015-08-12
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation methods of hydroxyapatite nanoparticles in the prior art are different, the morphological characteristics are different, the properties are different, and the anti-tumor effect is uneven.
Most of the nanoparticles produced by the general method in the prior art have no anti-tumor effect

Method used

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  • Hydroxyapatite nanoparticle with antitumor activity, preparation method and application thereof
  • Hydroxyapatite nanoparticle with antitumor activity, preparation method and application thereof
  • Hydroxyapatite nanoparticle with antitumor activity, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1, Preparation and Characterization of Hydroxyapatite Nanoparticles HAPN-1

[0074] Weigh 18.89 g Ca(NO 3 ) 2 and 6.34 g (NH 4 ) 2 HPO 4 , were dissolved in 400mL and 240mL of high-purity water, and the two raw materials were prepared into 0.2M aqueous solution. Put the prepared solution into a 4°C refrigerator for pre-freezing, then place the pre-frozen solution in an ice-water bath, and mix the above (NH 4 ) 2 HPO 4 Ca(NO 3 ) 2 Mix in the solution, and adjust the pH of the reaction system to 10-12 with ammonia water; then stir and react overnight in an ice-water bath, and keep the pH>10 during the reaction. After the reaction is complete, the reaction solution is centrifuged to collect the prepared particles, and the particles are alternately washed with high-purity water and ethanol for 3 times; then placed in a freeze dryer for 24 hours; finally, the sample is placed in a muffle furnace at 600°C After calcining for 2 hours, hydroxyapatite nanoparti...

Embodiment 2

[0080] Example 2, Preparation and Characterization of Hydroxyapatite Nanoparticles HAPN-2

[0081] Weigh 18.89 g Ca(NO 3 ) 2 and 6.34 g (NH 4 ) 2 HPO 4 , were dissolved in 400mL and 240mL of high-purity water to prepare a 0.2M aqueous solution. The prepared solution was preheated in a 40°C water bath, and then the preheated solution was placed in a 40°C water bath, and the above (NH 4 ) 2 HPO 4 Ca(NO 3 ) 2Mix in the solution, and adjust the pH of the reaction system to 10-12 with ammonia water; then stir and react overnight in a water bath at 40°C, and keep the pH>10 during the reaction. After the reaction is complete, the reaction solution is centrifuged to collect the prepared particles, and the particles are alternately washed with high-purity water and ethanol for 3 times; then placed in a freeze dryer for 24 hours; finally, the sample is placed in a muffle furnace at 600°C Calcined for 2 hours to obtain hydroxyapatite nanoparticles (referred to as HAPN-2).

[0...

Embodiment 3

[0084] Example 3, Preparation and Characterization of Base Apatite Nanoparticles HAPN-3

[0085] Weigh 18.89 g Ca(NO 3 ) 2 and 6.34 g (NH 4 ) 2 HPO 4 , were dissolved in 400mL and 240mL of high-purity water to prepare a 0.2M aqueous solution. The prepared solution was preheated in a 60°C water bath, and then the preheated solution was placed in a 60°C water bath, and the above (NH 4 ) 2 HPO 4 Ca(NO 3 ) 2 Mix in the solution, and adjust the pH of the reaction system to above 10 with ammonia water; then stir and react overnight in a water bath at 60°C, and keep the pH>10 during the reaction. After the reaction is complete, the reaction solution is centrifuged to collect the prepared particles, and the particles are alternately washed with high-purity water and ethanol for 3 times; then placed in a freeze dryer for 24 hours; finally, the sample is placed in a muffle furnace at 600°C Calcined for 2 hours, the hydroxyapatite nanoparticles were obtained, denoted as HAPN-3....

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Abstract

The invention relates to a hydroxyapatite nanoparticle with antitumor activity, a preparation method and application thereof. The invention discloses the near-spherical nano-hydroxyapatite with a particle size of 10-100nm, amorphous state and uniform particle distribution. The nanoparticle has very excellent antitumor activity and a tumor cell inactivation rate up to about 80%. The hydroxyapatite nanoparticle is prepared by a liquid phase precipitation method. The invention also discloses the use of the nanoparticle, and a pharmaceutical composition and a medicine box containing the nanoparticle.

Description

technical field [0001] The invention belongs to the field of inorganic materials and medicine, and more specifically, the invention relates to hydroxyapatite nanoparticles with antitumor activity, its preparation method and application. Background technique [0002] Hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 Hydroxyapatite (HAP for short) is the main inorganic component in human bone and tooth tissue, because of its adsorption, high binding, excellent biocompatibility, weak cytotoxicity, and no immune antigenicity to most protein and nucleic acid molecules. , non-tumorigenicity, relatively good loading transfection ability and controlled release (Sergey V.Dorozhkin, Biomaterials., 2010, 31:1465-1485; He LH, et al.ActaBiomaterials2008; 4:577-86) , has been widely cited in the field of biomedicine, such as bioimplantation materials, bone damage repair, drug carriers, antibacterial materials, immune sensors, and gene therapy. As a material with good biocompatibility and safe d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/42A61K9/14A61P35/00
Inventor 刘昌胜钱江潮袁媛陈雅莹孙懿
Owner EAST CHINA UNIV OF SCI & TECH
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