Preparation method of green-fluorescence-labeled terbium/strontium codoped hydroxyapatite nano particles with high luminous intensity

A technology of hydroxyapatite and luminous intensity, which is applied in nanotechnology, nanotechnology, luminescent materials, etc. for materials and surface science. It can solve the problems of fluorescence quenching and high rare earth content, and achieve reduced usage and high economy. benefit, cost reduction effect

Inactive Publication Date: 2017-07-28
SHAANXI UNIV OF SCI & TECH
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  • Description
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Problems solved by technology

[0004] However, conventional rare earth ion-doped hydroxyapatite fluorescent probes

Method used

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  • Preparation method of green-fluorescence-labeled terbium/strontium codoped hydroxyapatite nano particles with high luminous intensity
  • Preparation method of green-fluorescence-labeled terbium/strontium codoped hydroxyapatite nano particles with high luminous intensity
  • Preparation method of green-fluorescence-labeled terbium/strontium codoped hydroxyapatite nano particles with high luminous intensity

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preparation example Construction

[0028] A method for preparing green fluorescent-labeled terbium / strontium co-doped hydroxyapatite nanoparticles with high luminous intensity, comprising the following steps:

[0029] (1) First, weigh a certain amount of Tb 4 o 7 Solid powder, add excess concentrated nitric acid solution to it, heat in a water bath at 85°C, add a small amount of deionized water after the nitric acid is fully volatilized, continue to evaporate until crystallization forms Tb(NO 3 ) 3 Crystals, prepared with deionized water and constant volume to obtain terbium nitrate solution, ready for use.

[0030] (2) Then, take a certain amount of calcium nitrate, strontium nitrate, diammonium hydrogen phosphate and sodium hydroxide and dissolve it with deionized water to form a solution. The terbium nitrate solution prepared in (1) and calcium nitrate solution, nitric acid The strontium solution is mixed evenly, and the diammonium hydrogen phosphate solution is slowly added dropwise to the above mixed so...

Embodiment 1

[0034] (1) First, weigh 0.56g of Tb 4 o 7 Solid powder, add excess concentrated nitric acid solution to it, heat in a water bath at 85°C, add a small amount of deionized water after the nitric acid is fully volatilized, continue to evaporate until crystallization forms Tb(NO 3 ) 3 Crystals, then prepared with deionized water and fixed to 0.5mol / L Tb 3+ Solution 6mL, ready to use.

[0035] (2) Prepare 90 mL of 0.5 mol / L calcium nitrate solution, 10 mL of 0.5 mol / L strontium nitrate solution, and 50 mL of 0.6 mol / L ammonium hydrogen phosphate solution with distilled water, and mix the above nitrate solutions (calcium nitrate solution, strontium nitrate solution and Terbium nitrate solution) mixed and stirred evenly. Then, the diammonium hydrogen phosphate solution is slowly added dropwise to the above mixed solution, placed on a magnetic stirrer and continuously stirred, and after being fully mixed, the pH value of the reaction system is adjusted to 14 with 2mol / L NaOH solut...

Embodiment 2

[0038] (1) First, weigh 0.56g of Tb 4 o 7 Solid powder, add excess concentrated nitric acid solution to it, heat in a water bath at 85°C, add a small amount of deionized water after the nitric acid is fully volatilized, continue to evaporate until crystallization forms Tb(NO 3 ) 3 Crystals, then prepared with deionized water and fixed to 0.5mol / L Tb 3+ Solution 6mL, ready to use.

[0039] (2) Prepare 70 mL of 0.5 mol / L calcium nitrate solution, 30 mL of 0.5 mol / L strontium nitrate solution, and 50 mL of 0.6 mol / L diammonium hydrogen phosphate solution with distilled water. Terbium nitrate solution) mixed and stirred evenly. Then, the diammonium hydrogen phosphate solution is slowly added dropwise to the above mixed solution, placed on a magnetic stirrer and continuously stirred, and after being fully mixed, the pH value of the reaction system is adjusted to 14 with 2mol / L NaOH solution. Stirring was continued for 4h after stabilization.

[0040] (3) After the stirring is...

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Abstract

The invention discloses a preparation method of green fluorescently labeled terbium/strontium co-doped hydroxyapatite nanoparticles with high luminous intensity. First, take Tb 4 o 7 Add excess concentrated nitric acid, heat in a water bath, and evaporate to form Tb(NO 3 ) 3 crystal, and then prepare terbium nitrate solution; secondly, calcium nitrate, strontium nitrate, diammonium hydrogen phosphate and sodium hydroxide are dissolved in deionized water to form a solution, and the terbium nitrate solution in (1) is mixed with calcium nitrate solution and strontium nitrate solution Evenly, add the diammonium hydrogen phosphate solution dropwise, adjust the pH value to 14 with sodium hydroxide solution after fully mixing, and fully stir to obtain solution A after the pH value is stable; finally, leave solution A to stand for aging, and then After washing, suction filtration, drying and grinding, green fluorescent-labeled Tb with high luminescence intensity can be obtained 3+ /Sr 2+ Co-doped with hydroxyapatite nanoparticles. The material prepared by the present invention compares Tb 3+ Doped with hydroxyapatite, the fluorescence intensity is higher, the color is more obvious, and the fluorescence lifetime is longer.

Description

technical field [0001] The invention relates to a hydroxyapatite nanoparticle with green fluorescent labeling performance, in particular to a preparation method of a green fluorescently labeled terbium / strontium co-doped hydroxyapatite nanoparticle with high luminous intensity. Background technique [0002] Fluorescent imaging materials play an important role in biomedical applications such as bioimaging, drug delivery, photodynamic therapy, and disease diagnosis and treatment. There are many kinds of commonly used fluorescent imaging materials, but organic fluorescent molecular materials are prone to photobleaching and photolysis, and semiconductor quantum dot materials have poor chemical stability and are toxic. Hydroxyapatite [Ca 10 (PO 4 ) 6 (OH) 2 , HA / HAp], is the main inorganic component in human and animal bone tissue, has excellent biocompatibility and biological activity. The structure of hydroxyapatite is hexagonal, and one of the most important features of i...

Claims

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Application Information

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IPC IPC(8): C01B25/32B82Y30/00B82Y40/00C09K11/71
CPCC01B25/325B82Y30/00B82Y40/00C01P2002/80C09K11/7753
Inventor 殷海荣刘晶李艳肖白建光张森唐元元王宇飞张攀
Owner SHAANXI UNIV OF SCI & TECH
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