Preparation method of spherical nano hydroxyapatite particles

Abstract

The invention discloses a preparation method of spherical nano hydroxyapatite particles. The preparation method comprises the following steps: (1) measuring a CQDs (carbon quantum dots) solution in a conical flask, regulating the pH value of the solution up to 10, and uniformly stirring at room temperature; (2) dropwisely adding a CaCl2 solution, and uniformly stirring; (3) dropwisely adding a Na2HPO4.12H2O solution, and standing at room temperature; (4) performing centrifugal separation on the prepared product, discarding the supernate, washing the underlayer product with distilled water and anhydrous ethanol respectively, then taking out the product, and drying in a drying oven to obtain khaki powder; and (5) calcining the powder in a muffle furnace, thereby removing carbon quantum dots to obtain white powder, namely HAP (hydroxyapatite) nanoparticles. According to the invention, the spherical hydroxyapatite nanoparticles are prepared at room temperature by using the carbon quantum dots as effective nucleation inducer through a chemical precipitation method. The hydroxyapatite prepared by the invention is quasi-spherical, and has the advantages of favorable dispersity, particle uniformity and high crystallinity. Besides, the preparation method has the advantages of simplicity, mild and controllable conditions and low cost.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a method for preparing novel spherical nano-hydroxyapatite particles. Background technique [0002] Hydroxyapatite (HAP), as the most stable calcium phosphate salt under physiological conditions, is the main inorganic mineral component of vertebrate bones and teeth. Due to its good biocompatibility and bioactivity, it is widely used in tissue engineering, bone repair and replacement, and dental materials. It has been confirmed that mesoporous HAP can promote new bone formation through osteoconduction mechanism without causing any Toxicity, inflammation and rejection. In addition, due to the unique chemical composition and crystal structure, HAP exhibits high affinity to biomolecules, such as DNA and RNA, and is therefore widely used as a controlled release carrier for proteins, drugs, genes, and growth factors. For example, as a drug carrier, nanoparticles can pass t...

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Problems solved by technology

Lu Yupeng et al. (CN1903706A) used calcium nitrate tetrahydrate (Ca(NO 3 ) 2 4H 2 O) and diammonium hydrogen phosphate ((NH 4 ) 2 HPO 4 ) as the raw material, the hydroxyapatite slurry was prepared by the chemical precipitation method, and then diluted with ammonium bicarbonate, stirred evenly, sprayed, combined with subsequent heat treatment, the hollow hydroxyapatite composed of nano-grains was obtained Microspheres, the prepared hydroxyapatite microspheres have uniform particle size, large surface area, high hollow rate, but low crystallinity and large particle size

However, until now, spherical nano-hydroxyapatite with regular morphology has been rarely reported.

Especially with carbon quantum dots as the core, there is no related report on spherical particles coated with hydroxyapatite

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