Dual-phase magnetic nano-composite scaffold material and preparation method thereof

A technology of magnetic nano and composite scaffolds, applied in the field of biomedical composite materials, to achieve the effects of facilitating adhesion growth and vascularization, reducing toxicity, and improving biomechanical properties

Inactive Publication Date: 2018-04-06
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Bone and cartilage tissue engineering currently mainly includes three important components: seed cells, cytokines and scaffold materials. The scaffold material is the cell carrier and is the most basic element, and its biological characteristics are required to promote the formation of bone and cartilage tissue. Repair; ideal scaffold materials should include the following points: ①Good biocompatibility and low cytotoxicity; ②Controllable degradability; ③Appropriate pore structure; ④Good mechanical properties, which can bear the mechanical load of bon

Method used

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  • Dual-phase magnetic nano-composite scaffold material and preparation method thereof
  • Dual-phase magnetic nano-composite scaffold material and preparation method thereof
  • Dual-phase magnetic nano-composite scaffold material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0046] Example 1

[0047] Step 1. Weigh 18.18g of PLGA and dissolve in 100ml of 1,4-dioxane, and stir it evenly in a 60°C water bath to obtain a homogeneous solution of the first PLGA and 1,4-dioxane;

[0048] Step 2. Weigh 1.82g of Col-I powder and add it to the homogeneous solution of the first PLGA and 1,4-dioxane obtained in step 1, ultrasonically shake for 20h, and electromagnetically stir for 16h to obtain a concentration of 20% by mass Cartilage phase scaffold solution;

[0049] Step 3. Weigh 16.66g of PLGA and dissolve in 100ml of 1,4-dioxane, and stir it evenly in a 60°C water bath to obtain a homogeneous solution of the second PLGA and 1,4-dioxane;

[0050] Step 4. Weigh 1.67g n-HA and 1.67g magnetic nanoparticles Fe 2 O 3 Add the second PLGA and 1,4-dioxane obtained in step 3 to the homogeneous solution of the second PLGA and 1,4-dioxane, ultrasonic vibration for 20 minutes, electromagnetic stirring for 16 hours, to obtain a concentration of 20% by mass and put it into the ...

Example Embodiment

[0053] Example 2

[0054] Step 1. Weigh 18.18g of PLGA and dissolve in 100ml of 1,4-dioxane, and stir it evenly in a water bath at 50°C to obtain a homogeneous solution of the first PLGA and 1,4-dioxane;

[0055] Step 2. Weigh 1.82g of Col-I powder and add it to the homogeneous solution of the first PLGA and 1,4-dioxane obtained in Step 1, ultrasonic vibration for 10h, electromagnetic stirring for 12h, to obtain a mass percentage concentration of 20% Cartilage phase scaffold solution;

[0056] Step 3. Weigh 16.66g of PLGA and dissolve in 100ml of 1,4-dioxane, and stir it evenly in a water bath at 50°C to obtain a homogeneous solution of the second PLGA and 1,4-dioxane;

[0057] Step 4. Weigh 1.67g n-HA and 1.67g magnetic nanoparticles Fe 2 O 3 , Adding the second PLGA and 1,4-dioxane obtained in step 3 to the homogeneous solution of the second PLGA and 1,4-dioxane, ultrasonic vibration for 10 minutes, electromagnetic stirring for 12 hours, to obtain a mass percentage concentration of ...

Example Embodiment

[0060] Example 3

[0061] Step 1. Weigh 18.18g of PLGA and dissolve in 100ml of 1,4-dioxane, and stir it evenly in a 70°C water bath to obtain a homogeneous solution of the first PLGA and 1,4-dioxane;

[0062] Step 2. Weigh 1.82g of Col-I powder and add it to the homogeneous solution of the first PLGA and 1,4-dioxane obtained in Step 1, ultrasonic vibration for 30h, electromagnetic stirring for 24h, to obtain a mass percentage concentration of 20% Cartilage phase scaffold solution;

[0063] Step 3. Weigh 16.66g of PLLA and dissolve in 100ml of 1,4-dioxane, and stir it evenly in a water bath at 70°C to obtain a homogeneous solution of the second PLGA and 1,4-dioxane;

[0064] Step 4. Weigh 1.67g n-HA and 1.67g magnetic nanoparticles Fe 2 O 3 Add the second PLGA and 1,4-dioxane obtained in step 3 to the homogeneous solution of the second PLGA and 1,4-dioxane, ultrasonic vibration for 30 minutes, electromagnetic stirring for 24 hours, to obtain a mass percentage concentration of 20% into...

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Abstract

The invention discloses a dual-phase magnetic nano-composite scaffold material. The dual-phase magnetic nano-composite scaffold material is prepared by compositing a cartilago phase with a bone phase,wherein the cartilago phase contains polylactic acid and a natural polymer compound; and the bone phase contains polylactic acid, nano-hydroxyapatite and magnetic nanoparticles. The invention furtherdiscloses a preparation method of the dual-phase magnetic nano-composite scaffold material. The three-dimensional dual-phase magnetic nano-composite scaffold material is prepared from polylactic acid, the natural polymer compound, nano-hydroxyapatite and the magnetic nano particles by virtue of a low-temperature rapid forming technique and is integrated with the advantages of the four materials,is capable of promoting the adhesion and proliferation of cells, reducing the toxicity of degradation products and improving the biomechanical properties based on good osteoconduction and biocompatibility and is relatively beneficial to the adhesion growth and vascularization of solid cells, and the speed and effect of the coalescence between artificial cartilages transplanted at bone defect partsof a joint cartilage and a subchondral bone and the bones are greatly increased and improved.

Description

technical field [0001] The invention belongs to the technical field of biomedical composite materials, and in particular relates to a dual-phase magnetic nanocomposite support material and a preparation method thereof. Background technique [0002] Clinically, articular cartilage damage caused by various diseases, such as arthritis or trauma, is often accompanied by damage to subchondral bone. Therefore, the joint repair of cartilage and subchondral bone is better than the repair of cartilage alone. Scaffolds in bone tissue engineering are mainly three-dimensional porous shapes, and need to meet cell adhesion, regulation and interaction between cells, have certain mechanical properties and release bioactive molecules, and can promote the temptation and differentiation of seed cells and proliferation, such as forming a microenvironment for the differentiation of bone marrow mesenchymal stem cells into chondrocytes and osteoblasts, and promoting the formation of cartilage and ...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/24A61L27/22A61L27/20A61L27/12A61L27/04A61L27/50
CPCA61L27/042A61L27/12A61L27/18A61L27/20A61L27/227A61L27/24A61L27/50A61L2430/02A61L2430/06C08L67/04C08L89/00C08L5/08
Inventor 黄江鸿王大平熊建义刘威陈洁琳段莉朱伟民尤微朱飞燕贾兆锋
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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