Method for preparing heparin collagen/chitosan porous rack of composite angiogenin
An angiogenin and porous scaffold technology is applied in the field of preparation of heparinized collagen/chitosan porous scaffolds, which can solve the problems of loss of biological activity and short half-life, and achieve the advantages of promoting vascularization, simple method and improving transplant survival rate. Effect
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Embodiment 1
[0028] Example 1: Effect of heparinization on microstructure of collagen / chitosan porous scaffold
[0029] The collagen of bovine tendon was extracted from bovine tendon by enzymatic hydrolysis and acid extraction. Collagen, chitosan are prepared into 0.5% solution respectively with the acetic acid solution of 0.5mol / l, then bovine tendon collagen solution and chitosan solution are mixed and injected into mould, and chitosan solution content (by weight) is 10% , freeze-dried at -20°C for 2h and then freeze-dried in a freeze dryer to obtain a collagen / chitosan porous scaffold; after the gained collagen / chitosan porous scaffold was treated at 105°C for 24h under vacuum, it was soaked in sodium heparin (purchased from In the 2-N-morpholine ethanesulfonic acid (50mmol / l) solution of SIGMA Company of the United States), the mass ratio of bovine tendon collagen to heparin sodium in the solution is 10:1, soak for 1h, and then add 1-ethyl-3 -3-(Dimethylaminopropyl)-carbodiimide solut...
Embodiment 2
[0030] Example 2: Effect of Heparinization on the Binding Rate of Angiogenin
[0031] The collagen of bovine tendon was extracted from bovine tendon by enzymatic hydrolysis and acid extraction. Collagen, chitosan are prepared into 0.5% solution respectively with the acetic acid solution of 0.5mol / l, then bovine tendon collagen solution and chitosan solution are mixed and injected into mould, and chitosan solution content (by weight) is 10% , freeze-dried at -20°C for 2h and then freeze-dried in a freeze dryer to obtain a collagen / chitosan porous scaffold; after the gained collagen / chitosan porous scaffold was treated at 105°C for 24h under vacuum, it was soaked in sodium heparin (purchased from In the 2-N-morpholine ethanesulfonic acid (50mmol / l) solution of SIGMA Company of the United States), the mass ratio of bovine tendon collagen to heparin sodium in the solution is 10:1, soak for 1h, and then add 1-ethyl-3 -3-(Dimethylaminopropyl)-carbodiimide solution (40mmol / l) and N-...
Embodiment 3
[0032] Example 3: Effects of Different Contents of Chitosan Solutions on the Binding Rate of Angiogenin in Heparinized Collagen / Chitosan Porous Scaffolds
[0033] The collagen of bovine tendon was extracted from bovine tendon by enzymatic hydrolysis and acid extraction. With the acetic acid solution of 0.5mol / l, collagen, chitosan are formulated into 0.5% solution respectively, then bovine tendon collagen solution and chitosan solution are mixed to obtain chitosan swelling liquid content (by weight) is respectively 5% , 10%, 20%, and 30% mixed solutions, freeze at -20°C for 2h and freeze-dry in a lyophilizer to obtain a collagen / chitosan porous scaffold; the obtained collagen / chitosan porous scaffold is treated at 105°C under vacuum for 24h Afterwards, it is immersed in the 2-N-morpholine ethanesulfonic acid (50mmol / l) solution that contains heparin sodium (purchased from U.S. SIGMA company), and the mass ratio of bovine tendon collagen and heparin sodium in the solution is 10...
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