Method for preparing heparin collagen/chitosan porous rack of composite angiogenin

An angiogenin and porous scaffold technology is applied in the field of preparation of heparinized collagen/chitosan porous scaffolds, which can solve the problems of loss of biological activity and short half-life, and achieve the advantages of promoting vascularization, simple method and improving transplant survival rate. Effect

Inactive Publication Date: 2006-03-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the half-life of angiogenin in the body is very short, and it will degrade within a few minutes and lose its biological activity. Therefore, the key to solving its application is to obtain a sustained release system for angiogenin.

Method used

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  • Method for preparing heparin collagen/chitosan porous rack of composite angiogenin
  • Method for preparing heparin collagen/chitosan porous rack of composite angiogenin
  • Method for preparing heparin collagen/chitosan porous rack of composite angiogenin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Effect of heparinization on microstructure of collagen / chitosan porous scaffold

[0029] The collagen of bovine tendon was extracted from bovine tendon by enzymatic hydrolysis and acid extraction. Collagen, chitosan are prepared into 0.5% solution respectively with the acetic acid solution of 0.5mol / l, then bovine tendon collagen solution and chitosan solution are mixed and injected into mould, and chitosan solution content (by weight) is 10% , freeze-dried at -20°C for 2h and then freeze-dried in a freeze dryer to obtain a collagen / chitosan porous scaffold; after the gained collagen / chitosan porous scaffold was treated at 105°C for 24h under vacuum, it was soaked in sodium heparin (purchased from In the 2-N-morpholine ethanesulfonic acid (50mmol / l) solution of SIGMA Company of the United States), the mass ratio of bovine tendon collagen to heparin sodium in the solution is 10:1, soak for 1h, and then add 1-ethyl-3 -3-(Dimethylaminopropyl)-carbodiimide solut...

Embodiment 2

[0030] Example 2: Effect of Heparinization on the Binding Rate of Angiogenin

[0031] The collagen of bovine tendon was extracted from bovine tendon by enzymatic hydrolysis and acid extraction. Collagen, chitosan are prepared into 0.5% solution respectively with the acetic acid solution of 0.5mol / l, then bovine tendon collagen solution and chitosan solution are mixed and injected into mould, and chitosan solution content (by weight) is 10% , freeze-dried at -20°C for 2h and then freeze-dried in a freeze dryer to obtain a collagen / chitosan porous scaffold; after the gained collagen / chitosan porous scaffold was treated at 105°C for 24h under vacuum, it was soaked in sodium heparin (purchased from In the 2-N-morpholine ethanesulfonic acid (50mmol / l) solution of SIGMA Company of the United States), the mass ratio of bovine tendon collagen to heparin sodium in the solution is 10:1, soak for 1h, and then add 1-ethyl-3 -3-(Dimethylaminopropyl)-carbodiimide solution (40mmol / l) and N-...

Embodiment 3

[0032] Example 3: Effects of Different Contents of Chitosan Solutions on the Binding Rate of Angiogenin in Heparinized Collagen / Chitosan Porous Scaffolds

[0033] The collagen of bovine tendon was extracted from bovine tendon by enzymatic hydrolysis and acid extraction. With the acetic acid solution of 0.5mol / l, collagen, chitosan are formulated into 0.5% solution respectively, then bovine tendon collagen solution and chitosan solution are mixed to obtain chitosan swelling liquid content (by weight) is respectively 5% , 10%, 20%, and 30% mixed solutions, freeze at -20°C for 2h and freeze-dry in a lyophilizer to obtain a collagen / chitosan porous scaffold; the obtained collagen / chitosan porous scaffold is treated at 105°C under vacuum for 24h Afterwards, it is immersed in the 2-N-morpholine ethanesulfonic acid (50mmol / l) solution that contains heparin sodium (purchased from U.S. SIGMA company), and the mass ratio of bovine tendon collagen and heparin sodium in the solution is 10...

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Abstract

The preparation process of porous heparinized collagen / chitosan rack with compounded angiogenin includes the following steps: dissolving ox tendon and chitosan separately in acetic acid solution to compound 0.5-5 % concentration solution, mixing chitosan solution in 5-30 % and ox tendon solution, molding and freeze drying to obtain porous collagen / chitosan rack; vacuum treatment, soaking the porous collagen / chitosan rack inside 2-N-morpholynyl ethane sulfonic acid solution of heparin sodium, treating with 1-ethyl-3-3-(dimethylaminopropyl)-carbonized diimine solution and N-hydroxy batanimide solution, washing, re-freezing to dry and to obtain porous heparinized collagen / chitosan rack; and soaking the porous heparinized collagen / chitosan rack inside angiogenin solution to obtain the porous heparinized collagen / chitosan rack with compounded angiogenin. The prepared rack has proper pore size and porosity and is used as the corium substitute in skin tissue engineering.

Description

technical field [0001] The invention relates to a preparation method of a heparinized collagen / chitosan porous scaffold compounded with angiogenin. Background technique [0002] The emergence of tissue-engineered skin provides a new therapeutic approach for the repair and healing of skin wounds. However, the survival rate of various tissue-engineered skin grafts is significantly lower than that of autologous split skin grafts. One of the main reasons is tissue engineering. The vascularization rate of skin transplantation is slower than that of autologous skin grafts, so accelerating the vascularization rate of tissue-engineered skin is the key factor to improve its survival rate. At present, the application of growth factors that can promote angiogenesis is a better way to promote vascularization of tissue engineered skin. [0003] Among many growth factors, angiogenin (angiogenin, ANG) is the only angiogenesis factor that has been discovered and isolated and purified based...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61F2/10
Inventor 韩春茂石海飞毛峥伟陈轶欣马列高长有
Owner ZHEJIANG UNIV
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