Bone-imitated hydroxyapatite-collagen composite scaffold and preparation method thereof

Abstract

The invention discloses a bone-imitated hydroxyapatite-collagen composite scaffold and a preparation method thereof. The bone-imitated hydroxyapatite-collagen composite scaffold comprises hydroxyapatite and collagen molecules, and the weight ratio of the hydroxyapatite to the collagen molecules is 1.7-2.3; the hydroxyapatite is of an ultrathin sheet structure, the length is 25-50 nm, the width is 10-30 nm, the thickness is 2-6 nm, and the maximum exposed crystal face is a (100) crystal face; and the 2theta value of the XRD spectrum is 30-35 degrees, the XRD spectrum shows a large envelope peak, and the Ca / P molar ratio of the XRD spectrum is 1.5-1.6. The collagen of the composite scaffold is of a triple helix structure and has biological activity; and the water absorption rate is 17.4-19.3 times of the self weight, the pore size is 50-300 [mu]m, the void ratio is as high as 98%-98.7%, and the compression modulus is as high as 0.84-0.9 MPa.

Description

technical field [0001] The invention belongs to the application in the field of bone tissue engineering materials and medical devices, and in particular relates to a bone-like hydroxyapatite-collagen composite bracket and a preparation method thereof. Background technique [0002] Bone defects caused by physical aging, congenital deformities, trauma infection, and tumor resection have constituted a huge clinical burden worldwide. At present, the commonly used treatments for bone defects in clinical practice include autologous bone, allogeneic bone transplantation, and implantation of bone tissue engineering materials. For many years, autologous bone transplantation has been considered the first choice for the treatment of bone defects. It has excellent biological advantages and mechanical properties, but the source of autologous bone is limited, which will cause secondary damage to patients, and there are still problems such as inflammation and infection after surgery. . B...

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Problems solved by technology

Chinese Patent Publication No. CN1526765A discloses "Composite Bone Tissue Engineering Scaffold Material and Its Preparation Method", which uses a direct mixing method to mix collagen and hydroxyapatite to prepare a composite scaffold. This patent is only a combination of hydroxyapatite and collagen Mechanical mixing can only be biomimetic from the composition; Chinese Patent Publication No. CN105597155A discloses "one-pot method for preparing hydroxyapatite-natural polymer nanocomposites", which adopts co-precipitation method to prepare hydroxyapatite- Natural polymer nanocomposites focus on the advantages of simple methods, mild reaction conditions, and low energy consumption. However, the main raw material used in this process is small molecule collagen, which does not have a triple helix structure, and its biological properties are also greatly reduced. Moreover, hydroxyapatite in the hydroxyapatite-natural polymer nanocomposite material prepared by this patent has a needle-like structure, and its ability to promote cell proliferation and differentiation is weaker than that of flaky hydroxyapatite with exposed (100) crystal planes; China Patent Publication No. CN105358189A discloses "Biomimetic Collagen-Hydroxyapatite Composite Material", in which fibrous collagen scaffolds are immersed in saturated Ca 2+ / H x PO 4 (3-x) The hydroxyapatite-collagen composite scaffold was prepared in a saturated aqueous solution of at least 24 hours. This patent adopts a simulated physiological environment immersion method for preparation. In addition, only a few patents adopt the in-situ mineralization process to prepare composite scaffolds, such as the "silk fibroin / nano-hydroxyapatite porous scaffold material and its preparation method" disclosed in Chinese Patent Publication No. CN102000362A. This patent mainly focuses on obtaining A scaffold material with high porosity, uniformly dispersed pores, interconnected pores, and significantly improved mechanical properties. It simply explores the improvement of mechanical properties of hydroxyapatite on scaffold materials, which is different from the focus of this patent

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