Bone-imitated hydroxyapatite-collagen composite scaffold and preparation method thereof

A technology of imitating bone hydroxyapatite and hydroxyapatite, which is applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of long process time, difficulty in industrialization, and lack of triple helix structure, etc., and achieve shortened reaction Time, easy to achieve, mild effect

A technology of imitating bone hydroxyapatite and hydroxyapatite, which is applied in medical science, prosthesis, tissue regeneration, etc., can solve the problems of long process time, difficulty in industrialization, and lack of triple helix structure, etc., and achieve shortened reaction Time, easy to achieve, mild effect

CN113117149AActive Publication Date: 2021-07-16BEIJING UNIV OF CHEM TECH
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  • Bone-imitated hydroxyapatite-collagen composite scaffold and preparation method thereof
  • Bone-imitated hydroxyapatite-collagen composite scaffold and preparation method thereof
  • Bone-imitated hydroxyapatite-collagen composite scaffold and preparation method thereof

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preparation example Construction

[0043] As yet another aspect of the present invention, a method for preparing a bone-like hydroxyapatite-collagen composite scaffold of the present invention includes reacting collagen-calcium ion solution and disodium hydrogen phosphate solution in situ in a high-gravity rotating bed to obtain a precursor After aging, washing, and freeze-drying, the bone-like hydroxyapatite-collagen composite material was obtained, and then homogenized, freeze-dried, cross-linked, and freeze-dried to obtain the bone-like hydroxyapatite-collagen composite scaffold. It specifically includes the following steps: in the following steps, the corresponding relationship between parts by volume and parts by mass is ml and g; specifically,

[0044] A method for preparing a bone-like hydroxyapatite-collagen composite scaffold of the present invention comprises the following steps:

[0045] 1) Dissolve the collagen sponge in aqueous acetic acid solution, add calcium nitrate tetrahydrate and stir for che...

Embodiment 1

[0066] A method for preparing a bone-like hydroxyapatite-collagen composite scaffold by using a hypergravity technique, comprising the steps of:

[0067] 1) Weigh 1 g of collagen sponge and dissolve it in 200 ml of 0.125 mol / l acetic acid aqueous solution in a constant temperature water bath at 20°C. After the collagen sponge is dissolved, add 3.5 g of calcium nitrate tetrahydrate solid, and stir at a speed of 1000 rpm for 5 min to make Ca 2+ Chelated with collagen. Weigh 3.185g of disodium hydrogen phosphate dodecahydrate as a solid, and dissolve it in 50ml of deionized water at room temperature;

[0068] 2) The collagen-calcium ion solution and the disodium hydrogen phosphate dodecahydrate solution are mixed and reacted for a certain period of time in a high-gravity rotating bed to obtain a mixed slurry. Among them, the high-gravity rotating bed is an external circulation rotating bed, the high-gravity factor is 285, the reaction temperature is 34°C, and the reaction time ...

Embodiment 2

[0074] Change the reaction temperature in Step 2 of Example 1 to 10°C and 20°C.

[0075] image 3 (RPB 10°C 20°C line) is the circular dichroism spectrum of the sample obtained in Example 2. Its positive absorption peak around 220nm and negative absorption peak around 205nm indicate that the collagen in the composite material has a triple helical structure.

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Abstract

The invention discloses a bone-imitated hydroxyapatite-collagen composite scaffold and a preparation method thereof. The bone-imitated hydroxyapatite-collagen composite scaffold comprises hydroxyapatite and collagen molecules, and the weight ratio of the hydroxyapatite to the collagen molecules is 1.7-2.3; the hydroxyapatite is of an ultrathin sheet structure, the length is 25-50 nm, the width is 10-30 nm, the thickness is 2-6 nm, and the maximum exposed crystal face is a (100) crystal face; and the 2theta value of the XRD spectrum is 30-35 degrees, the XRD spectrum shows a large envelope peak, and the Ca / P molar ratio of the XRD spectrum is 1.5-1.6. The collagen of the composite scaffold is of a triple helix structure and has biological activity; and the water absorption rate is 17.4-19.3 times of the self weight, the pore size is 50-300 [mu]m, the void ratio is as high as 98%-98.7%, and the compression modulus is as high as 0.84-0.9 MPa.

Description

technical field [0001] The invention belongs to the application in the field of bone tissue engineering materials and medical devices, and in particular relates to a bone-like hydroxyapatite-collagen composite bracket and a preparation method thereof. Background technique [0002] Bone defects caused by physical aging, congenital deformities, trauma infection, and tumor resection have constituted a huge clinical burden worldwide. At present, the commonly used treatments for bone defects in clinical practice include autologous bone, allogeneic bone transplantation, and implantation of bone tissue engineering materials. For many years, autologous bone transplantation has been considered the first choice for the treatment of bone defects. It has excellent biological advantages and mechanical properties, but the source of autologous bone is limited, which will cause secondary damage to patients, and there are still problems such as inflammation and infection after surgery. . B...

Claims

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Application Information

Patent Timeline
16 Jul 2021
Publication
CN113117149A
IPC
A61L27/46; A61L27/50
CPC
A61L27/46; A61L27/50; A61L2430/02; A61L2430/12; C08L89/00
Inventors
乐园; 陈月亮