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Novel coronapneumonia virus rapid detection test strip and application thereof

A technology for detecting pneumonia virus and test strips, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of difficult operation for clinical medical staff, unfavorable early diagnosis, etc., and achieve home or on-site detection without auxiliary reagents and instruments , without the effect of drawing blood

Active Publication Date: 2020-06-12
WUXI PEOPLES HOSPITAL
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AI Technical Summary

Problems solved by technology

[0006] Serological IgM / IgG antibody ELISA detection is mainly to understand the recent infection status of the patient by detecting the IgM antibody of the new coronavirus pneumonia virus in the serum, but it needs to be completed by blood drawing, and IgM / IgG usually only appears after 2 weeks of infection, which is not conducive to early diagnosis, and It brings certain difficulties to the operation of clinical medical staff

Method used

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  • Novel coronapneumonia virus rapid detection test strip and application thereof

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Comparison scheme
Effect test

Embodiment 1

[0047] First of all, the solid-phase melt-blown polypropylene membrane (like N95 membrane) with ACE2 receptor activity can block the virus on the membrane, clean air can be filtered, and the virus blocked on the membrane can be blocked by the ACE2 receptor. Capture to form ACE2 receptor and virus solid-phase conjugates.

[0048] Afterwards, the immobilized membrane was drip-washed with lysis washing solution (reagent A) to promote viral RNA exposure.

[0049] Then, a specific probe (B reagent) complementary to the viral RNA sequence is added, and the probe is labeled with a chromogenic group or a biological enzyme to form a trimolecular complex of ACE2 receptor-virus-probe.

[0050] Finally, the immobilized membrane was drip-washed with buffer (C reagent) to remove non-specifically bound probes.

[0051] The detection device directly detects the color development or the color development of the enzyme substrate. If the development or color development is positive for the viru...

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Abstract

The invention discloses a novel coronapneumonia virus rapid detection test strip and application thereof, and belongs to the field of biotechnology detection. Specifically, the novel coronapneumonia virus is firstly combined with an immobilized N95-like membrane with ACE2 receptor activity; an ACE2 receptor-virus-probe trimolecular compound is formed by adding a specific probe with complementary virus RNA sequences, and due to the fact that a chromogenic reagent is marked on the probe, chromogenic means that viruses are positive, and non-chromogenic means that viruses are negative. The kit hasthe characteristics of simpleness, rapidness, no need of blood drawing, capability of realizing home or field detection, specificity, sensitivity, no need of auxiliary reagents and instruments, result observation by naked eyes and the like, and becomes the fastest, simplest and most convenient novel coronapneumonia virus detection technology at present.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a test strip for rapid detection of novel coronavirus pneumonia virus and its application. Background technique [0002] The new type of coronavirus pneumonia virus coronavirus is a single-stranded positive-sense RNA virus without segments, belonging to the Nidovirales (Nidovirales) Coronaviridae (Coronaviridae) positive coronavirus subfamily (Orthocoronavirinae), according to the serotype and genomic characteristics of the coronavirus subfamily The family is divided into four genera α, β, γ and δ. There are 6 coronaviruses known to infect humans, including 229E and NL63 of the genus α, OC43 and HKU1 of the genus β, Middle East respiratory syndrome-associated coronavirus (MERSr-CoV) and severe acute respiratory syndrome-associated coronavirus (SARSr-CoV). CoV). The coronavirus this time is a new type of coronavirus belonging to the genus Beta. [0003] Coronavirus...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569G01N33/543
CPCG01N33/68G01N33/56983G01N33/54306G01N2333/165Y02A50/30
Inventor 陆培华邹健陈卫东
Owner WUXI PEOPLES HOSPITAL
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