RNA silencing in animals as an antiviral defense

a technology of rna silencing and animal body, applied in animal repellents, peptide sources, biocide, etc., can solve the problems of ineffective co-transfection with dsrnas targeting mrnas of the two i>drosophila /i>dicer genes, and achieve the effect of inhibiting the expression of a candidate gene, enhancing the antiviral rna silencing pathway, and treating or preventing
US20060094055A1Inactive Publication Date: 2006-05-04RGT UNIV OF CALIFORNIA
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
RGT UNIV OF CALIFORNIA
Publication Date
2006-05-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides recombinant DNA constructs for inactivation of viral or endogenous genes in a cell, wherein the construct comprises viral sequence sufficient to activate RNA silencing. In another aspect, the invention provides methods for identifying RNA silencing suppressors by sequence analysis and functional tests. In yet another aspect, the invention provides a method for identifying inhibitors of RNA silencing suppressors. In still other aspects, the invention comprises methods for identifying genes in the antiviral RNA silencing pathway, enhancers of the antiviral pathway, and methods of treating or preventing viral infections using enhancers of the pathway.
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Description

STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT

[0001] This work was made with Government support under Grant No.2002-35319-11537. awarded by the U.S. Department of Agriculture. The government has certain rights in this invention.CROSS-REFERENCES TO RELATED APPLICATIONS

[0002] NOT APPLICABLE REFERENCE TO A “SEQUENCE LISTING,” A TABLE, OR A COMPUTER PROGRAM LISTING APPENDIX SUBMITTED ON A COMPACT DISK

[0003] NOT APPLICABLE BACKGROUND OF THE INVENTION

[0004] RNA interference (RNAi) is a process where introduction of dsRNA into a cell causes destruction of RNA in a sequence-specific manner (see, D. Baulcombe, Curr. Biol., 12:R83 (2002); Hutvagner et al., Curr. Opin. Genet. Dev., 12:225 (2002)) RNAi has been observed in plants, Neurospora, flies, protozoans, and mice. Available data show that double-stranded (ds) RNA serves as the initial trigger of RNA interference and upon recognition, is processed by the Dicer RNAse into short fragments of...

Claims

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