Preparation method of targeting porphyrin fluorescent molecule and gold nanorod dyad

A technology of fluorescent molecules and gold nanorods, which is applied in the field of preparation of doublets, can solve the problems that phototoxic gold nanorods cannot exist stably, poor targeting, etc., so as to improve the targeting ability and tumor treatment effect and improve the effect. , the effect of reducing side effects in normal tissues

Inactive Publication Date: 2013-02-27
HARBIN INST OF TECH
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Problems solved by technology

[0006] The purpose of the present invention is to solve the technical problems that porphyrin-based fluorescent molecules are poorly targeted and easily aggregated in the skin and eyes to produce phototoxicity and that gold nanorods cannot exist stably in polar solvents when they are transported in the body. Preparation method of targeted porphyrin-based fluorescent molecule and gold nanorod duplex

Method used

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  • Preparation method of targeting porphyrin fluorescent molecule and gold nanorod dyad
  • Preparation method of targeting porphyrin fluorescent molecule and gold nanorod dyad
  • Preparation method of targeting porphyrin fluorescent molecule and gold nanorod dyad

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specific Embodiment approach 1

[0022] Specific embodiment 1: The preparation method of the targeted porphyrin fluorescent molecule and gold nanorod duplex in this embodiment is carried out according to the following steps:

[0023] 1. Mix 10-100mg porphyrin-based fluorescent molecules, 2-5g anhydrous potassium carbonate and 15-30ml N,N-dimethylformamide under the condition of avoiding light and 0°C for 5-10 minutes, and then Add 1-5ml of chloroacetyl chloride dropwise within ~40 minutes, continue to react for 8-12 hours, remove potassium carbonate in the product, add 50ml of water and 50ml of dichloromethane to the product, take out the dichloromethane solution by separation, and pour it into the dichloromethane Add water to the methane solution and extract 3 to 5 times, then evaporate the solvent in the dichloromethane solution to dryness, and then separate by column chromatography, and use a mixed solution of dichloromethane and methanol with a volume ratio of 100:2~3 as eluting Liquid, collect the second...

specific Embodiment approach 2

[0043] Embodiment 2: This embodiment differs from Embodiment 1 in that the porphyrin-based fluorescent molecules described in step 1 are tetrahydroxyphenylporphyrin, trihydroxyphenylporphyrin, and tetracarboxyphenylporphyrin. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0044] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is that in step 1, 50 mg of porphyrin-based fluorescent molecules, 5 g of anhydrous potassium carbonate and 20 ml of N, N-dimethyl The formamide mixture was stirred for 8 minutes. Others are the same as in the first embodiment.

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Abstract

The invention relates to a preparation method of dyad, particularly relates to a preparation method of targeting porphyrin fluorescent molecule and gold nanorod dyad and solves technical problems that porphyrin fluorescent molecules are prone to gather on the skin and eyes to generate phototoxicity due to poor targeting when the porphyrin fluorescent molecules are transported in bodies and gold nanorods can not stably exist in polar solvents. The method includes preparing a golden seed solution, preparing a gold nanorod growing solution, preparing a polyethylene glycol stabilized gold nanorod solution, preparing the dyad, mixing biomolecules or anticarcinogen and the dyad with N, N-dimethylformamide for reaction, performing centrifugation, dispersing precipitates in methyl alcohol, and then performing separation again to obtain the targeting porphyrin fluorescent molecule and gold nanorod dyad. According to the prepared targeting porphyrin fluorescent molecule and gold nanorod dyad, effects of early-stage tumor diagnosis and treatment can be improved, side effects on normal organizations can be reduced, and the dyad can be connected with biomacromolecules and the anticarcinogen which have targeting actions so that targeting capacity and tumor treatment effects are further improved.

Description

technical field [0001] The invention relates to a method for preparing a doublet. Background technique [0002] Malignant tumor is a disease with a high mortality rate, and early diagnosis and treatment are key scientific issues in tumor research. In tumor diagnosis technology, compared with positron emission tomography, magnetic resonance imaging, ultrasound and computer tomography, optical imaging has attracted the attention of researchers due to its advantages of low energy radiation and high sensitivity. The basic principle is to add exogenous fluorescent probes that can be enriched in tumor tissues, and then use a certain wavelength of light to excite the probes to generate fluorescence, and then diagnose by monitoring the difference in fluorescence signals between tumor tissues and normal tissues; optical imaging The core is the intensity of light absorption and the level of fluorescence quantum yield of fluorescent probes. Porphyrin fluorescent molecules have a rigi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K41/00A61K47/48A61P35/00
Inventor 郭喜明薄璇郭斌
Owner HARBIN INST OF TECH
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