Preparation method of targeting porphyrin fluorescent molecule and gold nanorod dyad

Abstract

The invention relates to a preparation method of dyad, particularly relates to a preparation method of targeting porphyrin fluorescent molecule and gold nanorod dyad and solves technical problems that porphyrin fluorescent molecules are prone to gather on the skin and eyes to generate phototoxicity due to poor targeting when the porphyrin fluorescent molecules are transported in bodies and gold nanorods can not stably exist in polar solvents. The method includes preparing a golden seed solution, preparing a gold nanorod growing solution, preparing a polyethylene glycol stabilized gold nanorod solution, preparing the dyad, mixing biomolecules or anticarcinogen and the dyad with N, N-dimethylformamide for reaction, performing centrifugation, dispersing precipitates in methyl alcohol, and then performing separation again to obtain the targeting porphyrin fluorescent molecule and gold nanorod dyad. According to the prepared targeting porphyrin fluorescent molecule and gold nanorod dyad, effects of early-stage tumor diagnosis and treatment can be improved, side effects on normal organizations can be reduced, and the dyad can be connected with biomacromolecules and the anticarcinogen which have targeting actions so that targeting capacity and tumor treatment effects are further improved.

Description

technical field [0001] The invention relates to a method for preparing a doublet. Background technique [0002] Malignant tumor is a disease with a high mortality rate, and early diagnosis and treatment are key scientific issues in tumor research. In tumor diagnosis technology, compared with positron emission tomography, magnetic resonance imaging, ultrasound and computer tomography, optical imaging has attracted the attention of researchers due to its advantages of low energy radiation and high sensitivity. The basic principle is to add exogenous fluorescent probes that can be enriched in tumor tissues, and then use a certain wavelength of light to excite the probes to generate fluorescence, and then diagnose by monitoring the difference in fluorescence signals between tumor tissues and normal tissues; optical imaging The core is the intensity of light absorption and the level of fluorescence quantum yield of fluorescent probes. Porphyrin fluorescent molecules have a rigi...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the technical problems that porphyrin-based fluorescent molecules are poorly targeted and easily aggregated in the skin and eyes to produce phototoxicity and that gold nanorods cannot exist stably in polar solvents when they are transported in the body. Preparation method of targeted porphyrin-based fluorescent molecule and gold nanorod duplex

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