Super-resolution differential interference phase contrast microscopic imaging system and microscopic imaging method

Abstract

The invention discloses a super-resolution differential interference phase contrast microscopic imaging system. The system comprises a microscope, a light source, a first lens, a first reflecting mirror, a spatial light modulator, a third lens, a light barrier, a fourth lens and a charge coupled device (CCD), and the microscope is provided with a differential interference phase contrast imaging module. Simultaneously, the invention further discloses a microscopic imaging method based on the imaging system. According to the imaging system and the imaging method, extra sample preparation processes are not required, photobleaching effects and phototoxicity effects are absent, and the imaging contrast ratio is high.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, in particular to a super-resolution differential interference phase contrast microscopic imaging system and imaging method based on structured light illumination. Background technique [0002] A super-resolution microscopic imaging system based on structured light illumination can improve the resolution of microscopic imaging. In the prior art, there are mainly two types of imaging systems. [0003] The first is an imaging system that applies structured light illumination to fluorescence microscopy imaging. It has good contrast, but its main problems are as follows: 1) To perform fluorescence imaging, it is necessary to perform fluorescence on the observed sample. 2) In the above sample preparation process, substances such as dyes or fluorophores need to be added to the sample, so some side effects may be introduced, such as photobleaching effect and phototoxicity effect. [0004] Th...

AI Technical Summary

Problems solved by technology

[0003] The first is an imaging system that applies structured light illumination to fluorescence microscopy imaging. It has good contrast, but its main problems are as follows: 1) To perform fluorescence imaging, it is necessary to perform fluorescence on the observed sample. 2) In the above sample preparation process, substances such as dyes or fluorophores need to be added to the sample, so some side effects may be introduced, such as photobleaching effect and phototoxicity effect

[0004] The second is a scattered light imaging system based on structured light illumination, which does not require fluorescent labeling of the sample, but its imaging contrast is low, often requiring the use of gold nanoparticles to assist imaging, or a sample with a certain contrast is required to achieve high Contrast imaging, resulting in poor applicability, for example, when imaging biological samples without any processing, it cannot be applied due to its low imaging contrast

Images