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Water-soluble up-conversion luminescence nano material based on triplet state-triplet state annihilation and preparation method and application thereof

A technology of nanomaterials and luminescent materials, applied in the application field of upconversion luminescence bioimaging, achieves the effects of high quantum yield, high sensitivity and excellent upconversion luminescence performance

Inactive Publication Date: 2013-09-11
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main limitations are the construction of water-soluble upconversion luminescence systems and the establishment of suitable imaging systems

Method used

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  • Water-soluble up-conversion luminescence nano material based on triplet state-triplet state annihilation and preparation method and application thereof
  • Water-soluble up-conversion luminescence nano material based on triplet state-triplet state annihilation and preparation method and application thereof
  • Water-soluble up-conversion luminescence nano material based on triplet state-triplet state annihilation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Material synthesis of upconversion luminescent SiO2 based on octaethylporphyrin palladium and 9,10 diphenylanthracene triplet annihilation.

[0051] Dissolve the octaethylporphyrin palladium and 9,10 diphenylanthracene together with polyoxyethylene-polyoxypropylene-polyoxyethylene in methylene chloride, and then volatilize methylene chloride to dryness under argon , then dissolved in 2mol / L hydrochloric acid aqueous solution, added tetraethyl orthosilicate, reacted for 48 hours, dialyzed to neutrality, obtained silicon-coated octaethylporphyrin palladium and 9,10 diphenylanthracene upconversion Glowing material.

[0052]

Embodiment 2

[0053] Example 2: SiO based on triplet triplet annihilation of tris-(4,4')-dimethylbipyridine and 9,10-dimethylanthracene 2 material synthesis.

[0054] The three-(4,4'-dimethyl) bipyridine nail [Ru(dmb) 3 ] 2+ and 9,10-dimethylanthracene DMA and polyoxyethylene-polyoxypropylene-polyoxyethylene were dissolved in dichloromethane, and then the dichloromethane was evaporated to dryness under argon, and then dissolved in 2 mol / L In the aqueous solution of hydrochloric acid, add ethyl orthosilicate, react for 48 hours, and dialyze to neutrality to obtain silicon-coated tris-(4,4'-dimethyl)bipyridine nail and 9,10-dimethyl Anthracene upconversion luminescent material. The obtained material can obtain blue upconversion luminescence under 514nm excitation.

[0055]

Embodiment 3

[0056] Example 3: Synthesis of polyacrylamide-modified polystyrene nanomaterials based on triplet-triplet annihilation of triphenylpyridine iridium and pyrene.

[0057] Emulsifier sodium dodecylbenzenesulfonate 150mg dissolved in 50mL deionized water, monomer styrene 2g and triphenylpyridine iridium Ir(ppy)3 and pyrene, initiator 2,2'-azobisisobutyronitrile (AIBN, 50mg) was added to the above solution, and the mixture was ultrasonically emulsified for 30 minutes to form a stable emulsion. The emulsion was poured into a three-necked bottle, degassed for 30 minutes, and then heated to <70°C for 24 hours. A small amount of acrylamide was added to the reaction. Centrifuge, disperse and wash with water, centrifuge and dry and store. The obtained material can obtain up-conversion emission in the range of 360-420nm under the excitation of 450nm.

[0058]

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Abstract

The invention belongs to the technical field of biological detection, and particularly relates to an up-conversion luminescence nano material based on triplet state-triplet state annihilation, a preparation method thereof and an application thereof in biological imaging. The invention provides an up-conversion luminescence material based on triplet state-triplet state annihilation, which can be applied to biological imaging. The up-conversion luminescence material based on triplet state-triplet state annihilation for biological imaging comprises a sensitizer and an annihilation agent, wherein the sensitizer is classified into three types; the first type of sensitizer is a porphyrin and phthalocyanine metal complex, and the metals in the complex include palladium, platinum, iridium and zinc; the second type of sensitizer is an iodo- or bromo-boron fluorine compound; and the third type of sensitizer is a complex of ruthenium, iridium and platinum. According to the material provided by the invention, the annihilation agent is a biphenyl and acene compound and a derivative thereof, or a bromo-boron fluorine compound or an imine luminous compound. The material has the advantages of high sensitivity, photobleaching resistance and the like, and has great application values in terms of biological marking and imaging.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to the preparation of up-conversion luminescence nanometer materials based on triplet-triplet annihilation and the application of up-conversion luminescence bioimaging. Background technique [0002] Fluorescence imaging technology uses fluorescent probes to mark the observation object, thereby realizing highly selective imaging of the observation object. In addition, fluorescence imaging also has the characteristics of non-ionized low-energy radiation, high sensitivity, and continuous real-time monitoring. It is a visualization method that can observe at the subcellular level. With the development of fluorescent probes for labeling various biomolecules and ions, and the emergence of laser scanning confocal fluorescence microscopy, researchers can perform three-dimensional imaging of living cells to realize the dynamic changes of fine structures and molecules...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N21/64A61K49/00
Inventor 李富友刘倩杨天赦
Owner FUDAN UNIV
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