Multichannel fluorescent microscopy composite microscopic system

Abstract

The invention provides a multichannel fluorescent microscopy composite microscopic system, and belongs to the technical field of microscopes. The multichannel fluorescent microscopy composite microscopic system comprises a microscope body, an imaging module and a composite lighting module. A mechanical rotating mirror is controlled to change the light path of the composite lighting module through a control system, and light matched with the imaging module and modulated through different modes is selected to enter the microscope body by the mechanical rotating mirror to perform lighting on a sample. The device has four functions including a two-color fluorescent localization microscopic function, a structured light lighting microscopic function, a function of fluorescent recovery after photobleaching and a bright field imaging function respectively. The appropriate method can be selected to perform high spatial resolution or high time resolution imaging on the sample in a bioexperiment according to the characteristics of the sample and the imaging requirements, and different modes of imaging can be performed on the same position of the same sample so that the problem of one-sidedness of the imaging result of the single microscopic method can be solved.

Description

technical field [0001] The invention belongs to the technical field of microscopes, in particular to a composite microscope system for multi-channel fluorescence microscopy. Background technique [0002] Biomicroscopy plays a pivotal role in the research of life science and technology, and with the development and refinement of research on life processes, biomicroscopy has encountered more and more challenges. The first challenge is that the scale of the observed content in the studied samples is getting smaller and smaller. The size of cells is on the order of ten microns, the size of organelles such as mitochondria and chloroplasts is on the order of microns, and the size of vesicles in cells is on the order of hundreds to several On the order of ten nanometers, the size of most proteins is less than 50 nanometers. To clearly see the life structure on these scales, the spatial resolution of the fluorescence microscope is highly required. The second challenge is the real-t...

AI Technical Summary

Problems solved by technology

The first challenge is that the scale of the observed content in the studied samples is getting smaller and smaller. The size of cells is on the order of ten microns, the size of organelles such as mitochondria and chloroplasts is on the order of microns, and the size of vesicles in cells is on the order of hundreds to several On the order of ten nanometers, the size of most proteins is less than 50 nanometers. To clearly see the life structure on these scales, the spatial resolution of the fluorescence microscope is very high.

The second challenge is the real-time observation of life processes, that is, the imaging of living samples. Because the time scale of life activities is on the order of seconds or milliseconds, the higher the time resolution of the fluorescence microscope, the more detailed life activities can be observed.

In the process of life science experiments, different fluorescent microscopes need to be selected for imaging different biological samples, which greatly increases the cost of research; replace the microscope system and find the same position, which is operationally difficult to do

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