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Method and system for determining fluorescence intensity of fluorescence image

A technology of fluorescence image and fluorescence intensity, applied in the field of cell detection

Active Publication Date: 2017-11-17
SHANGHAI RUIYU BIOTECH
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Problems solved by technology

[0003] However, image analysis based on microscopic fluorescence imaging and intelligent recognition expresses fluorescence intensity based on pixels and grayscales, which is completely different from flow cytometry, which uses voltage pulses to express fluorescence intensity

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  • Method and system for determining fluorescence intensity of fluorescence image
  • Method and system for determining fluorescence intensity of fluorescence image
  • Method and system for determining fluorescence intensity of fluorescence image

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Embodiment Construction

[0014] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0015] In practical applications, flow cytometry provides a variety of indicators for customers to choose from, allowing customers to analyze target cells from different sides. The main indicators are: 1. The corresponding lateral fluorescence channel collected when the cells pass through The cumulative voltage pulse value was used as the cumulative fluorescence intensity. 2. The strongest voltage pulse corresponding to the lateral fluorescence channel collect...

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Abstract

The invention provides a method and system for determining the fluorescence intensity of a fluorescence image. The method includes the steps that light field imaging and fluorescence imaging are conducted on a target sample by adopting a microscopic fluorescence imaging system to obtain the light field image and the fluorescence image, edge extraction and segmentation are conducted on a fluorescence image region of each detection target in the fluorescence image, and the fluorescence image region corresponding to each detection target in the fluorescence image is obtained; the cumulative gray value, the maximum gray value, and the average gray value of the fluorescence image region of each detection target, and the diameter value of the light field image region of each detection target are calculated, streaming clustering analyses are conducted based on the cumulative gray value, the maximum gray value, the average gray value and the light field diameter value. Analyses can be conducted by executing or inputting into a streaming clustering analysis software, and corresponding and similar results of the streaming analysis are obtained.

Description

technical field [0001] The invention relates to the technical field of cell detection, in particular to a method and system for determining the fluorescence intensity of a fluorescence image. Background technique [0002] Flow cytometry is an important cell analysis technology, and its core principle is to perform clustering analysis on the fluorescence intensity of detection targets (cells or other detection particles). The flow cytometry technique expresses the fluorescence intensity by recording the voltage pulse value of the fluorescence signal. Now, with the development of microscopic fluorescence imaging technology and intelligent recognition technology, by performing fluorescence imaging on a large number of detection targets and analyzing them through image recognition methods, cluster analysis based on fluorescence imaging technology can be realized. Analysis-based fusion flow clustering analysis method (image-like flow analysis method) has become a new analysis me...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
CPCG01N15/1431G01N15/1434G01N2015/144G01N2015/1493G01N15/1433G06V20/69
Inventor 颜海波黄海清罗浦文
Owner SHANGHAI RUIYU BIOTECH
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