The invention discloses a near-
infrared light field three-dimensional imaging
system and application, and the
system is based on single-
photon imaging and comprises an illumination light path and a
light field imaging light path. The illumination light path generates an illumination
light beam of a near-
infrared band, and the illumination
light beam is projected on a sample by adopting vertical illumination to excite
fluorescence of the sample in a Rayleigh range of the illumination
light beam; a
light spot in the Rayleigh range of the illumination light beam is elliptical; a
galvanometer is arranged in the illumination light path, and a tube lens is arranged between the
galvanometer and the objective lens, so that an illumination light beam scans in an imaging view field in parallel to a main
optical axis of the objective lens; the
light field imaging light path is provided with a
microlens array, collects
fluorescence emitted by the sample, records the
fluorescence direction to obtain light field information, and performs light field three-dimensional imaging on the sample. According to the invention, selective illumination is realized by shaping the illumination beam of the near-
infrared band, the
imaging quality, the imaging depth and the imaging
speed of light field three-dimensional imaging are considered in combination with
beam scanning, and the method is suitable for real-time three-dimensional imaging of a
living body.