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Plant cytoplasmic male sterile recovering gene and its use

A male sterility and cytoplasmic technology, applied in the direction of plant gene improvement, application, plant peptides, etc., to shorten the breeding time and improve the efficiency of breeding selection

Inactive Publication Date: 2003-06-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the application of the present invention was filed, there was no report on the cloning of the male sterility restoration gene of rice

Method used

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  • Plant cytoplasmic male sterile recovering gene and its use
  • Plant cytoplasmic male sterile recovering gene and its use
  • Plant cytoplasmic male sterile recovering gene and its use

Examples

Experimental program
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Embodiment 1

[0038] Example 1. Fine mapping and cloning of rice restorer gene Rf1

[0039] In the present invention, the Rf1 gene is cloned by a map-based cloning method. First, the rice Baotai cytoplasmic male sterile line 731A was crossed with the restorer line C9083 to establish a F 2 Separate groups of individuals. According to the reported approximate position of the Rf1 locus on rice chromosome 10 (Ichikawa, 1997), molecular markers in this region were selected for polymorphism detection and linkage analysis. These markers include Y3-8 (Zhang Qunyu et al., 2002), C1361, S10019 (RGP Research Institute of Japan: http: / / rgp.dna.affrc.go.jp), and the BAC clone sequence OSJNBa0078O01 according to the data of the rice genome sequence database The polymorphic probes 01-2.5, 01-2, and Rf1m were amplified by PCR using the primers designed and synthesized at the position. Linkage analysis was performed with these markers, and a linkage map of the Rf1 locus region was constructed ( figure ...

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Abstract

The present invention provides the nucleotide sequence and encoded amino acid polypeptide sequence of one cytoplamis male sterile restoring gene OsRf1 originating from rice. The gene is member of PPR gene family, and is one constitutive expression gene. The present invention also relates to the application of utilizing the gene in transforming rice and other plant breeding restorer and the application of the molecular label produced based on the gene sequence in breeding.

Description

technical field [0001] The present invention relates to the cloning and application of plant genes, in particular to the DNA sequence and the encoded protein sequence of rice cytoplasmic male sterility restorer gene obtained by cloning. The present invention further relates to the use of restorer genes in plant breeding. Background technique [0002] Plants produce hybrids through cross-pollination between different species or different varieties. Hybrids have strong advantages in growth vigor, adaptability, yield, etc., called heterosis. The use of heterosis can improve the yield, quality and resistance of crops. The phenomenon that the male reproductive organs of plants cannot produce normal functioning male gametes, that is, pollen, is called male sterility. Plant or crop varieties with the characteristic of male sterility are called sterile lines. On the other hand, some plants or crop varieties are crossed with sterile lines to produce hybrids with normal fertility. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00C07H21/00C07K14/415C12N15/29C12N15/82C12Q1/68
Inventor 刘耀光王中华吴豪张群宇苏弟华陈乐天
Owner SOUTH CHINA AGRI UNIV
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