Method for breeding hybrid seeds of rape of heterogenesis cytoplasm male sterility line

A male sterile line and male sterile technology, applied in the field of plant bioengineering, can solve problems such as unstable fertility, decreased purity of hybrid seed production, and PolCMS is easily affected by temperature, achieving high induction success rate and hybrid selection Easy, stable and fast results

Inactive Publication Date: 2007-10-03
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pol CMS is easily affected by temperature, and the instability of fertility leads to a decrease in the purity of hybrid seed production. The exis

Method used

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  • Method for breeding hybrid seeds of rape of heterogenesis cytoplasm male sterility line
  • Method for breeding hybrid seeds of rape of heterogenesis cytoplasm male sterility line
  • Method for breeding hybrid seeds of rape of heterogenesis cytoplasm male sterility line

Examples

Experimental program
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Effect test

Embodiment 1

[0076] Implementation Example 1: Intergeneric hybridization creates male sterility in Brassica napus containing wild mustard cytoplasm

[0077] 1. Use plant material to carry out protoplast fusion or sexual hybridization (see implementation example 2) to obtain a somatic cell hybrid containing wild mustard cytoplasm. The plant material used is Brassica napus double-low variety Zhongshuang 4 or Xinjiang wild mustard resource wild oil 18 number, including the following steps:

[0078] A., Isolation of mesophyll protoplasts: after surface disinfection (put 70% ethanol for 1 minute, 1.5% sodium hypochlorite for 10 minutes, rinse 3 times with sterile water), the seeds of Brassica napus and wild mustard are inoculated in MS On the medium, set 25oC, 16 hours 60-80μmol m -2 the s -1 Grow under light conditions. Fully expanded leaves were used for the isolation of protoplasts. During enzymatic hydrolysis, 3.5ml of SCM solution (0.5M sorbitol, 10mM CaCl2, 5mM MES, pH5.8) was added t...

Embodiment 2

[0120] Example 2: Obtaining Somatic Cell Hybrids Containing Mustard Cytoplasm by Sexual Crossing

[0121] 1) Ovary culture to produce intergenus hybrids containing wild mustard cytoplasm: plant wild mustard and Brassica napus in the field, take wild mustard as female parent and Brassica napus as male parent in flowering stage, carry out artificial emasculation and pollination, after pollination 7-10 days, harvest Back to the siliques that have begun to develop, after surface disinfection (putting 70% ethanol for 1 minute, 1.5% sodium hypochlorite for 10 minutes, and rinsing with sterile water for 3 times), put the MS medium containing 0.8% hydrolyzed casein 60-80μmol m at 25°C for 16 hours -2 the s -1 Cultivate under light conditions. After 35-40 days, take out the immature embryos in the siliques and germinate them on MS medium. After germination, 60-80 μmol m -2 the s -1 Let it grow true leaves under light conditions.

[0122] 2) Chromosome doubling of intergenus hybrids: ...

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Abstract

A method for selectively culturing the allogenic cytoplasm male sterility line of rape includes such steps as separating the protoplast from leaf pulp, purifying, pre-treating, fusion, culturing, discriminating hybrid, reproduction, and continuous backcross to obtain sterility line and maintenance line.

Description

technical field [0001] The invention relates to interspecies hybridization technology to recombine the cytoplasm and nucleus genome of rape to create heterologous cytoplasm male sterility, which belongs to the field of plant bioengineering. The invention more specifically relates to a method for breeding heterologous cytoplasmic male sterile lines of rapeseed, which can be used to establish heterologous cytoplasmic male sterile rapeseed with different types of cytoplasm, and to prepare high-yielding hybrids in breeding. Background technique [0002] The Brassica cultivars used for seed harvesting and oil extraction mainly include Brassica napus, Chinese cabbage rape, mustard rape and Ethiopian mustard. The other two Brassica cultivars, Chinese cabbage and Brassica oleracea, are mainly used as vegetables. The use of heterosis to increase yield is the main way of high-yielding breeding of these cultivars. [0003] Cytoplasmic male sterility in rapes...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H1/06A01H4/00A01H5/00C12Q1/68C12N5/14
Inventor 胡琼李云昌梅德圣李英德徐育松方小平刘胜毅程计华
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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