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Method for breeding allied hybrid species utilizing anther of Capsicum hybrid species

A technology for hybrids and peppers, which is applied in the field of breeding for similar hybrids, and can solve problems such as separations that have not yet been seen

Inactive Publication Date: 2003-05-21
北京海花生物科技有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The advantage is that the breeding speed is fast and the breeding efficiency is improved. Therefore, the anther culture technology is widely used in the field of breeding. A report on close hybrids of pepper hybrids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] Embodiment: a rectangular bell pepper hybrid (No. 97-1001) introduced from abroad.

[0048] 97-1001 was planted in the greenhouse in 1997. Buds were taken when the plants were budding. After the buds were sterilized, the anthers were removed and inoculated on SPD solid medium for cultivation. The temperature was maintained at 35°C for the first 7 days. Maintain a constant temperature of about 28°C to 30°C. Under 1500 lux light, 10 hours of light per day.

[0049] When the capsicum haploid seedlings grown on the medium grew to 6 leaves, the seedlings were removed and rinsed, then transplanted on the sterilized soil, and when the transplanted seedlings grew 3 new leaves, the It is transplanted to garden soil in due course. When the seedling grows to 10 leaves and grows normally and vigorously, press the absorbent cotton ball tightly on the growth point of the seedling, and add a mixture of 0.1% to 0.2% colchicine and 2.0% dimethyl sulfoxide to the cotton ball Doubling so...

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Abstract

The method for cultivating approximate hybrid species by utilizing anther of hot-pepper hybrid species includes the following steps: 1. sowing hot-pepper hybrid seed, in bud stage of plant taking budand making anther culture; 2. utilizing anther culture to obtain haploid plant, and utilizing chromosome doubling to obtain double haploid line of original hot-pepper hybrid species; 3. in several double haploid lines, according to main economic trait and approximate property of original hybrid species, selecting parental line which can be used for original hot-pepepr hybrid species and breaking several hot peper hybrid species; and 4. comparing, testing and breeding approximate hybrid species which has no clear difference from original hot-pepper hybrid species in main economic trait.

Description

Technical field: [0001] The invention belongs to a breeding method for plant hybrids, and more particularly relates to a breeding method for screening the parents of pepper hybrids by adopting anther culture technology and then breeding similar hybrids. Background technique: [0002] At present, the capsicum hybrid breeding technology widely used at home and abroad is a conventional breeding technology, which generally includes the following steps: first, several capsicum inbred lines (lines) are selected and bred, and then two inbred lines are artificially paired and pollinated. Hybrid combination, and then through a series of selective breeding process, bred hybrids that can be popularized and applied in production. [0003] Anther culture technology is a breeding technology developed in the 1960s, and has become an important part of modern biotechnology. The main point of this technology is to culture the anthers of hybrids in vitro, induce pollen to differentiate into h...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 李春玲邢永萍张树根蒋钟仁
Owner 北京海花生物科技有限公司
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