Cucumber male sterility gene, molecular marker, screening method and application thereof

Abstract

The invention relates to SNP and InDel markers related to a cucumber male sterility gene and a screening method thereof. The cucumber male sterility gene is acquired by screening according to the markers. Specifically, the screening method includes following steps: (1), preparing groups; (2), building a library, and sequencing; (3), adopting bulked segregation analysis (BSA); (4), performing competitive allele specificity PCR (polymerase chain reaction) (KASP), finely mapping a sterility gene, and acquiring SNP or InDel locus in close linkage with sterility character. An effective tool is provided for quick identification of cucumber male sterility plants and efficient production of cucumber hybrid species by acquiring the male sterility gene.

Description

technical field [0001] The invention relates to the technical field of molecular genetic breeding, in particular to SNP or InDel markers related to cucumber male sterility genes, screening thereof, obtained male sterility genes and their use in identifying male sterile plants. Background technique [0002] At present, many male sterility genes have been located in other crops, and many markers closely linked to male sterility genes have been developed, but there are few reports on male sterility genes in cucumber, let alone their application. In addition, nuclear-cytoplasmic interaction sterile lines, photosensitive sterile lines and temperature-sensitive sterile lines are the most used at present, and the use of nuclear sterile genes is seldom, mainly because it is difficult to find maintainer lines. The traditional method of gene mapping is to use the existing molecular markers to isolate a large number of screened differential bands in the population, and the positioning ...

AI Technical Summary

Problems solved by technology

[0002] At present, many male sterility genes have been located in other crops, and many markers closely linked to male sterility genes have been developed, but there are few reports on male sterility genes in cucumbers, let alone applications.

In addition, nuclear-plasma interaction sterile lines, photosensitive sterile lines, and temperature-sensitive sterile lines are the most used at present, and the use of nuclear sterile genes is seldom, mainly because it is difficult to find maintainer lines.

Traditionally, the method of gene mapping is to use existing molecular markers to isolate a large number of screening differential bands in the population, and the positioning of genes is too time-consuming and labor-intensive.

[0003] Although the patent application CN105420408A explored the molecular marker of cucumber sterility gene, it only obtained one SNP marker, and the SNP marker was too single to locate the cucumber sterility gene

Images