Chlorella polysaccharide extraction method

An extraction method and chlorella technology, applied in the field of marine biology, can solve the problems of not being widely used, narrow extraction range and high operating cost, and achieve the effects of promoting rapid dissolution, avoiding nutrient loss and low production cost

Inactive Publication Date: 2015-11-25
都宝君
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the equipment is complicated, the operating cost is high,

Method used

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  • Chlorella polysaccharide extraction method
  • Chlorella polysaccharide extraction method
  • Chlorella polysaccharide extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Pretreatment: Weigh 100g of chlorella powder and dissolve it in 500ml of distilled water to prepare a suspension with a concentration of 5%, put it in a 60°C water bath for 1 hour and keep stirring;

[0016] 2. Freeze-thaw and ultrasonic treatment: freeze the chlorella suspension at ~20°C and thaw at room temperature. Repeat the freeze-thaw process 4 times, freezing for 12 hours each time; then add 3 mL of phosphate buffer solution with a pH of 7.0 and a concentration of 0.01 mol / L, sonicate (400 W, time 4 min), and centrifuge in an ultracentrifuge at 2000 r / min for 5 min. Filtrate, the filtrate is standby;

[0017] 3. Enzymatic hydrolysis of the filter residue: add 200ml of distilled water to the filter residue in step 2 to prepare a 10% solution, add 1.0ml of trypsin, enzymolysis temperature 60°C, enzymolysis time 2h, centrifuge, speed control 4000r / min, time 10min, Filtrate, the filtrate is standby;

[0018] 4. Concentration under reduced pressure and alcohol an...

Embodiment 2

[0046] 1. Pretreatment: Weigh 200g of chlorella powder and dissolve it in 1000ml of distilled water to prepare a suspension with a concentration of 5%, put it in a water bath at 60°C for 1 hour and keep stirring;

[0047] 2. Freeze-thaw and ultrasonic treatment: freeze the chlorella suspension at ~20°C and thaw at room temperature. Repeat the freeze-thaw process 4 times, freezing for 12 hours each time; then add 5 mL of phosphate buffer solution with a pH of 7.0 and a concentration of 0.01 mol / L, sonicate (500 W, time 5 min), centrifuge at 2000 r / min for 5 min, and filter. The filtrate is ready for use;

[0048]3. Enzymatic hydrolysis of the filter residue: add 300ml of distilled water to the filter residue in step 2 to prepare a 15% solution, add 1.5ml of trypsin, enzymolysis temperature 55°C, enzymolysis time 2h, centrifuge, speed control 3500r / min, time 10min, Filtrate, the filtrate is standby;

[0049] 4. Concentration under reduced pressure and alcohol analysis: combine...

Embodiment 3

[0053] 1. Pretreatment: Weigh 50g of chlorella powder and dissolve it in 500ml of distilled water to prepare a suspension with a concentration of 10%, put it in a water bath at 60°C for 1 hour and keep stirring;

[0054] 2. Freeze-thaw and ultrasonic treatment: freeze the chlorella suspension at ~20°C and thaw at room temperature. Repeat the freezing and thawing process 4 times, freezing for 12 hours each time; then add 4.5 mL of phosphate buffer solution with a pH of 7.0 and a concentration of 0.01 mol / L, ultrasonicate (600 W, time 6 min), centrifuge at 2500 r / min for 5 min, filter , the filtrate is standby;

[0055] 3. Enzymatic hydrolysis of the filter residue: add 100ml of distilled water to the filter residue in step 2 to prepare a 20% solution, add 0.5ml of trypsin, enzymolysis temperature of 55°C, enzymolysis time of 2h, centrifuge at a speed of 4000r / min for 10min, Filtrate, the filtrate is standby;

[0056] 4. Concentration under reduced pressure and alcohol analysi...

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Abstract

The invention discloses a chlorella polysaccharide extraction method, and relates to the technical field of marine organisms. Cell walls are completely broken through alternate freezing and thawing; meanwhile, the bad influences that nutrition of raw materials is lost and effective components of the raw materials are seriously destroyed due to high temperature are avoided by combining an ultrasonic-assistant extraction method, and polysaccharides in chlorella cells can be promoted to be quickly dissolved out. Compared with a traditional polysaccharide extraction method, the chlorella polysaccharide extraction method has the advantages of being low in energy consumption, high in polysaccharide extraction rate and recovery rate and low in production cost. According to the method of combining alternate freezing and thawing with ultrasonic extraction, the chlorella polysaccharide extraction rate is 1.34-1.39 times of that of the traditional extraction method, and the recovery rate is 1.1-1.58 times of that of the traditional extraction method.

Description

technical field [0001] The invention relates to the technical field of marine organisms, in particular to a method for extracting chlorella polysaccharides. Background technique [0002] Chlorella (Chlorella spp.) is a kind of universal unicellular green algae, belonging to Chlorphyta (Chlorphyta) Chlorophyceae (Chlo-rophyceae) Chlorococcales (ChlorocOccales) Oocystaceae (Oocystaceae) Chlorella genus ( Chlorella). There are as many as 15 species of chlorella known in the world. Chlorella is rich in bioactive substances, such as unsaturated fatty acids, pigments, proteins, polysaccharides, etc. It has anti-tumor, anti-bacterial and anti-viral properties, and can prevent and treat peptic ulcer and iron-deficiency anemia, anti-hyperlipidemia and atherosclerosis It has various functions such as hardening, anti-radiation, detoxification, liver protection and lowering blood pressure. Chlorella has the advantages of wide ecological distribution, rapid growth and reproduction, an...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 都宝君
Owner 都宝君
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