Identification method of channel catfish species

A technology for channel catfish and identification methods, which is applied in the direction of microbial determination/inspection, biochemical equipment and methods, and can solve problems such as cumbersome operation process, lack of mitochondrial DNA molecular data, and unsuitability for rapid and economical identification of channel catfish species. , to achieve the effect of short time and simple operation
CN103409516AInactive Publication Date: 2013-11-27罗志萍
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
罗志萍
Publication Date
2013-11-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an identification method of channel catfish species. The identification method comprises following steps: genome DNA of channel catfish muscular tissues is extracted; a PCR primer is designed in mitochondrial DNA D-loop region; the genome DNA is subjected to PCR amplification with the presence of the PCR primer so as to obtain mitochondrial DNA D-loop sequences; the mitochondrial DNA D-loop sequences are subjected to enzyme digestion for 2 to 3h at a temperature of 37 DEG C by using restriction enzyme EcoR I; and then the mitochondrial DNA D-loop sequences are subjected to agarose gel electrophoresis after enzyme digestion, and the channel catfish species are identified according to the results of electrophoresis. Enzyme sites are searched based on sequencing of D-loop, and enzyme digestion identification is performed, so that sequencing is not necessary for identification of channel catfish species. The identification method is capable of identifying channel catfish species at a molecular level; operation is simple and easy; consumed time is short; and identification method is suitable for species identification of channel catfish in import and export trade.
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Description

technical field

[0001] The invention relates to a method for identifying channel catfish species, in particular to a method for identifying genetic information of channel catfish species by using a mitochondrial DNA D-loop control region, and belongs to the field of molecular biological PCR genetic identification. Background technique

[0002] Channel catfish (Ictalurus punctatus), also known as Channel Catfish, belongs to Siluriformes, Ictaluridae, and Ictalurus. It is native to North America, and its natural range is in the central United States, southern Canada, and northern Mexico. Channel catfish is a rare and valuable freshwater economic fish, and is deeply loved by consumers in the United States, Canada and many other countries. Processed finished products or semi-finished products are very popular in the markets of the United States, Japan, Europe and Canada, and the market demand is huge. Since 1984, my country’s Hubei Provincial Fisheries Research Institute intro...

Claims

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