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Extraction process for collagen of channel catfish skin

A technology of channel catfish and channel catfish, which is applied in the direction of animal/human protein, peptide preparation, connective tissue peptide, etc., can solve the problems of chemical reagent residue, product quality reduction, environmental non-point source pollution, etc., to achieve product safety, Save labor and production cost, simple equipment effect

Inactive Publication Date: 2009-12-23
湖北省水产科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The skin of channel catfish is rich in collagen, but the processing enterprises in my country do not carry out intensive processing at present. Channel catfish is directly processed into fish skin by-products for consumption and sale, which has low economic value and waste of resources; very few processing enterprises use acid-base, etc. Extraction of channel catfish skin collagen with organic solvents not only causes non-point source pollution to the environment, low product purity and chemical reagent residues reduce product quality, but also can cause side effects on the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The embodiment of the present invention gets 500 grams of channel catfish skin raw material, is 0.025% edible alkali (Na 2 CO 3 ) solution and raw materials were mixed in a ratio of 10:1 to treat the non-collagen protein on the raw material of channel catfish skin. The treatment method was to stir for 8 hours at a low temperature of 3°C, wash it twice with clear water, and then use a concentration of 2.0% The edible brine was agitated for 8 hours at a low temperature of 4°C, washed twice with clean water, and then decolorized with 0.15% edible hydrogen peroxide and agitated for 4 hours at a low temperature of 4°C. Wash the decolorized channel catfish skin with water, dry the water and mince it, adjust the pH value to 5.5, add 0.35 grams of papain at a ratio of 0.7‰ of the weight of the raw material substrate, and cool at 4°C Under the condition of heat preservation and hydrolysis for 30 hours, the liquid after enzymatic hydrolysis is deactivated by vacuum filtration of...

Embodiment 2

[0032] The embodiment of the present invention gets 500 grams of channel catfish skin raw material, is 0.02% edible alkali (Na 2 CO 3 ) solution and the raw material are mixed in a ratio of 15:1 to process the non-collagen protein on the channel catfish skin raw material. The processing method is to stir for 5 hours under the low temperature condition of 4° C., wash cleanly for 2 times with clear water, and then use a concentration of 1.8% The edible brine was agitated for 5 hours at a low temperature of 4°C, washed twice with clean water, and then decolorized with 0.11% edible hydrogen peroxide and agitated for 3 hours at a low temperature of 4°C. Wash the decolorized channel catfish skin with water, dry the water and mince it, adjust the pH value to 6.0, add 0.5 g of papain at a ratio of 1.0 ‰ of the raw material substrate weight, and cool at 3°C Under the condition of heat preservation and hydrolysis for 35 hours, the liquid after enzymatic hydrolysis is deactivated by vac...

Embodiment 3

[0034] The embodiment of the present invention gets 500 grams of channel catfish skin raw material, is 0.033% edible alkali (Na 2 CO 3 ) solution and the raw material are mixed in a ratio of 15:1 to process the non-collagen protein on the channel catfish skin raw material, and the processing method is to stir for 8 hours at a low temperature of 5°C, wash it twice with clear water, and then use a concentration of 2.2% The edible brine was agitated for 9 hours at a low temperature of 4°C, washed twice with clean water, and then decolorized with 0.13% edible hydrogen peroxide and agitated for 2 hours at a low temperature of 4°C. Wash the decolorized channel catfish skin with water, dry the water and mince it, adjust the pH value to 5.0, add 0.75 grams of papain at a rate of 1.5‰ of the raw material substrate weight, and cool at 6°C Keep warm for 40 hours under the same conditions, the enzyme hydrolyzed liquid is deactivated by 215KU vacuum membrane vacuum filtration, and then di...

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PUM

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Abstract

The invention discloses an extraction process for collagen of channel catfish skin, which comprises the following steps: firstly, impure protein of channel catfish skin scrape is removed, then channel catfish skin is decolorized, and finally breaking treatment, enzyme hydrolysis, enzyme inactivation, concentration treatment, low-temperature drying and grinding treatment are carried out on channel catfish skin to obtain the finished products. The extraction process finely and further processes and comprehensively utilizes the channel catfish skin scrape; the purity of extracted collagen II type product can reach more than 90%; the effect of scrape additional value is specially obvious; the product has wide usage scope; the whole extraction process does not have heavy metal proof, residual medicine, is healthy and safety; after being processed, the product does not have plane-source environment and water source pollution; and the extraction process flow and the device needed by the process flow are simple and have low manufacturing cost.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a process for extracting collagen by using leftover fish skin produced during channel catfish processing as a raw material. technical background [0002] Collagen is a structural protein with multiple biological functions. Collagen is the support and filler of tissues, actively participates in cell migration, differentiation and proliferation, and is related to wound repair and embryonic development. Bio-substitutes prepared from collagen have the functions of moisturizing, repairing, anti-aging, and nutrition, so they are widely used in wound and burn repair, plastic surgery, nerve regeneration, and blood valve surgery. [0003] Collagen used in the past was mainly extracted from mammalian pig and cowhide. The pre-treatment of this type of collagen extraction is cumbersome. In order to obtain clean skin raw materials, the workload required for cleaning...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/14
Inventor 蔡焰值雷晓中
Owner 湖北省水产科学研究所
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