Artificial insemination method for yellow-head catfish in hypotonic solution

Abstract

The invention discloses an artificial insemination method for a yellow-head catfish in a hypotonic solution, and belongs to aquaculture technologies. The artificial insemination technology is finished by innovating the way of acquiring the sperms and the eggs of the yellow-head catfish, designing a sperm storage solution and pre-activating the sperms in the hypotonic solution. The artificial insemination method adopts the following technical scheme: the artificial insemination method comprises the steps as follows: (1) filtering spermaries taken out after the male fish is bled in a bolting silk in an extruding way to obtain spermatic fluid, and refrigerating in the storage solution for storage to improve the sperm acquisition quality, wherein the spermatic fluid of each male fish can be provided for artificial insemination of 7.5 kg of the eggs; (2) extruding the eggs by two fingers to reduce the visceral injury of the female fish and increase the survival rate after delivery; and (3) taking the hypotonic solution with the concentration of 0.2-0.4% as an insemination medium instead of water, pre-activating the sperms in the hypotonic solution and inseminating with the eggs to enhance the sperm activity and the controllability of artificial insemination are enhanced, increase the insemination rate of artificial insemination and improve the stability of artificial insemination. The artificial insemination method is stable and reliable, and is suitable for large-scale production and fishes with homogeneous egg types.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and relates to the key technology of artificial propagation of yellow catfish, in particular to an artificial insemination method of yellow catfish in a hypotonic solution. Background technique [0002] The production of seedlings of farmed fish is generally obtained through natural reproduction and artificial reproduction, while large-scale production mainly relies on artificial reproduction; in the process of artificial reproduction, the fertilization rate is the key technical indicator of production, and affects the fertilization rate. In addition to the quality of eggs and sperm, the artificial insemination method is also an important factor affecting the stability of the fertilization rate; the current conventional artificial insemination methods, whether it is wet insemination or dry insemination, the fertilization of eggs and sperm The process is completed after the synchronous activat...

AI Technical Summary

Problems solved by technology

[0002] The seedling production of farmed fish is generally obtained through natural reproduction and artificial reproduction, while large-scale production mainly relies on artificial reproduction; in the process of artificial reproduction, the fertilization rate is the key technical indicator of production, and affects the fertilization rate. In addition to the quality of eggs and sperm, the artificial insemination method is also an important factor affecting the stability of the fertilization rate; the current conventional artificial insemination methods, whether it is wet insemination or dry insemination, the fertilization of eggs and sperm The process is completed after the synchronous activation of the water medium. For fish with strong sticky eggs, such as yellow catfish, the eggs are easy to stick to the water during the artificial insemination process. It will not only affect the operation of artificial insemination, but also affect the Stability of fertilization rate, lower hatch rate;

[0003] At present, the breeding of yellow catfish seedlings is also obtained through natural spawning in ponds or artificial propagation. Although the production cost of natural spawning is relatively low, due to the influence of natural spawning on climate, water temperature and other factors, the asynchrony of spawning and Unstable, its production scale is difficult to meet the requirements of large-scale production; and during the artificial reproduction of yellow catfish, the female and male fish are not in sync, and the conventional artificial insemination method needs to collect eggs and semen simultaneously, so the process of egg collection is easy to squeeze Injury to viscera leads to high postnatal mortality of female fish. Male fish need to kill the male fish to obtain sperm. The sperm is obtained by grinding or cutting the testis, which is easily polluted by blood clots and other tissues brought in. The sperm storage time is short, and the male fish wastes a lot and is fertilized. The process time is strictly controlled, and the eggs are easy to form agglomerates after contact with water, which affects the artificial insemination operation and hatching rate; production stability