Hybridization breeding method of undaria pinnatifida and sea tangle

Abstract

The invention relates to a method adopting undaria pinnatifida female gametophytes saved and cultured indoors and sea tangle female and male gametophytes to be mutually used as male parents and female parents and cutting the gametophytes. The method comprises the following steps of: mixing according to the mode of the undaria pinnatifida female gametophytes * the sea tangle male gametophytes and the sea tangle female gametophytes * undaria pinnatifida male gametophytes; cutting into algae segments of 200 micrometers by a tissue pounding machine, and then scattering on a vinylon seeding bed; and cultivating the vinylon seeding bed at temperature suitable for the growth and the maturity of the undaria pinnatifida and sea tangle gametophytes, wherein after culturing for 7 days to 10 days, the sea tangle female gametophytes are mature to form oocysts and ovulate ova which are respectively fertilized with sperms released by mature sea tangle and undaria pinnatifida male gametophytes so as to germinate hybrid seedlings. The invention can sufficiently utilize the heteroses of the distant hybridization of undaria pinnatifida and sea tangle; in addition, obtained hybrids have inheritable characteristics of the undaria pinnatifida and the sea tangle, high stress resistance, fast growth and good quality and can greatly increase the cultivation output of the hybrid undaria pinnatifida and sea tangle and enhance the quality of the hybrid undaria pinnatifida and sea tangle.

Description

Technical field: [0001] The invention relates to a hybrid breeding method between families, especially a method with simple operation and low cost, which can make full use of the heterosis of distant hybridization of wakame and kelp, and can greatly improve the cultivation yield and quality of hybrid wakame and kelp Wakame and kelp hybrid breeding method. Background technique: [0002] Wakame (Undaria pinnatifida) belongs to Phaeophyta, Brownia, Laminaria, Pteraceae, and Undaria genus. It belongs to euthermic algae. It is delicious and nutritious. , sugar and vitamins, because the cellulose content of wakame is only about 3% of the dry weight, it is easy to be digested by the human body, and it is a healthy food popular among consumers. [0003] Kelp (Laminaria japonica) belongs to Phaeophyta, Phaeophyta, Laminaria, Laminariaceae, and Laminaria genus in classification, and belongs to cold-water algae. Because kelp is rich in protein, sugar and vitamins, it has the function...

AI Technical Summary

Problems solved by technology

[0004] At present, the production of wakame seedlings mainly includes indoor full-artificial seedling cultivation and artificial spore-harvesting semi-artificial seedling cultivation in sea areas. Among them, the method of spore collection is mainly used in the production of artificial seedling production, and small-scale gametophyte collection is also carried out. Indoor seedling cultivation ; Kelp is natural light and low temperature full artificial seedling cultivation, mainly adopts the spore picking seedling method; what the genetic breeding of the two all adopted is the method for intraspecific hybridization or control ploidy to carry out germplasm improvement, so far there is no information on the inter-species relationship between Undaria pinnatifida and Laminariaceae. Related reports on hybrid breeding