Saccharina japonica female gametophyte specific molecular marker FSMSJ-1294 and application thereof

Abstract

The invention discloses a saccharina japonica female gametophyte specific molecular marker FSMSJ-1294 and application thereof, and belongs to the field of genetic engineering. A sequence of the saccharina japonica female gametophyte specific molecular marker FSMSJ-1294 is shown as SEQ ID NO: 5, sequences of amplification primers of the saccharina japonica female gametophyte specific molecular marker FSMSJ-1294 are shown as SEQ ID NO: 3 and SEQ ID NO: 4, and the saccharina japonica female gametophyte specific molecular marker FSMSJ-1294 can be used for identifying a parental origin of a parthenogenetic saccharina japonica sporophyte and the gender of offspring of the parthenogenetic saccharina japonica sporophyte. A cell line cloned by female and male gametophytes of saccharina japonica is cultured, gametophyte cells are collected, genome DNA of the gametophyte cells is extracted, PCR amplification is carried out by using the primers to obtain an amplification product, and after electrophoresis detection, if a specific band with the length of 1294bp can be detected, the cell line is determined as a saccharina japonica female gametophyte cell line. The saccharina japonica female gametophyte specific molecular marker FSMSJ-1294 is stable in identification result, rapid and convenient to operate, high in stability, and low in detection cost.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular to FSMSJ-1294, a specific molecular marker for female gametophytes of laminaria, and its application, which are used for the identification of parental sources of parthenogenetic laminar sporophytes and the sex of offspring. Background technique [0002] Kelp (Saccharina japonica) belongs to Phaeophyta and Laminariales. As early as the end of the 1970s, my country began to establish the cloning (cloning) technology of kelp gametophytes (Fang Zongxi et al., 1978). Using this technology, the female and male gametophytes of kelp can be preserved independently for a long time, so it is a germplasm resource of excellent traits in kelp. the best form of preservation. However, in the process of germplasm preservation and seedling breeding of Laminaria gametophytes, the mixture of female and male gametophytes is usually caused by incomplete separation of female and male gametophytes by mi...

AI Technical Summary

Problems solved by technology

There are few studies on the SDR of kelp, but in species such as Undaria pinnatifida and Macrocystis pyrifera, which are closely related to kelp and belong to the Phytophyta, Lipinska et al. (2015) based on Ectocarpus siliculosus ) gene sequence at the U chromosome SDR, and developed female gametophyte-specific molecular markers of Wakame and macroalgae, indicating that the gene located at the U chromosome SDR of Longsac Shuiyun is relatively conserved

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