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Method for increasing proportion of drynaria roosii female gametophytes

A technology of female gametophytes and spores of Quercus fern, applied in the field of increasing the ratio of female gametophytes of Quercus fern

Active Publication Date: 2016-07-27
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no scientific research institution or company in China to conduct research on obtaining parthenogenetic female gametophytes of ferns

Method used

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  • Method for increasing proportion of drynaria roosii female gametophytes
  • Method for increasing proportion of drynaria roosii female gametophytes
  • Method for increasing proportion of drynaria roosii female gametophytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Effect of LED blue light treatment on the differentiation of gametophyte sex organs of Quercus fern

[0025] Pour the sterilized 1 / 2MS medium into a 35mm disposable petri dish for sowing the spores of Quercus fern. After the medium is solidified, sow the spores of Quercus fern, seal with a sealing film, and culture under an incandescent lamp At the end of the filament stage, they were cultured under LED blue light at the lamellar body stage, heart-shaped prothallus formation stage, and sexual organ differentiation stage (5 biological replicates at each stage), and the rest of the stages were cultured under incandescent lamps. , after the sex organs were completely differentiated, the gametophytic differentiation was counted.

[0026] Table 1

[0027]

[0028] The results showed that compared with the control, the LED blue light treatment of the flake stage, heart-shaped prothallus stage, and sexual organ differentiation stage was beneficial to the formatio...

Embodiment 2

[0029] Example 2 Effect of LED red light treatment on the differentiation of gametophyte sex organs of Quercus fern

[0030] Pour the sterilized 1 / 2MS medium into a 35mm disposable petri dish for sowing the spores of Quercus fern. After the medium is solidified, sow the spores of Quercus fern, seal with a sealing film, and culture under an incandescent lamp At the end of the filamentous stage, they were cultured under LED red light at the flaky body stage, heart-shaped prothallus formation stage, and sexual organ differentiation stage (5 biological replicates at each stage), and the rest of the stages were under incandescent lamps. After culturing, the differentiation of gametocytes was counted after the sex organs were completely differentiated.

[0031] Table 2

[0032]

[0033] The results showed that LED red light treatment was beneficial to the formation of parthenogenetic female gametophytes (83.3%) in the formation stage of heart-shaped prothallus, but there were 16...

Embodiment 3

[0034] Example 3 Effect of LED green light treatment on the differentiation of gametophyte sex organs of Quercus fern

[0035] Pour the sterilized 1 / 2MS medium into a 35mm disposable petri dish for sowing the spores of Quercus fern. After the medium is solidified, sow the spores of Quercus fern, seal with a sealing film, and culture under an incandescent lamp At the end of the filamentous stage, they were cultured under LED green light at the flaky body stage, heart-shaped prothallus formation stage, and sexual organ differentiation stage (5 biological replicates for each stage), and the rest of the stages were under incandescent lamps. After culturing, the differentiation of gametocytes was counted after the sex organs were completely differentiated.

[0036] table 3

[0037]

[0038] Result analysis: LED green light treatment was beneficial to the formation of the heart-shaped prothallus and sexual organ differentiation stages, and produced more asexual gametophytes; LED...

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Abstract

The invention discloses a method for increasing the proportion of drynaria roosii female gametophytes. The method comprises the steps that after drynaria roosii spores are sown aseptically, then placed under an incandescent lamp to be cultured to form protonemata, placed under LED blue light or LED red light or LED white light or LED green light at a plate stage, a heart-shaped prothallium formation stage or a sexual organ differentiation stage respectively to be cultured and placed under the incandescent lamp at the other stages. Compared with other LED light qualities, a large proportion (80%) of unisexual female gametophytes are generated when the LED blue light is preferentially selected for treatment in the heart-shaped prothallium formation stage, and the other 20% gametophytes are asexual, so that the selfing behavior in the breeding process is avoided, and the method is the quite ideal light quality treatment mode for obtaining the unisexual female gametophytes.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for increasing the ratio of female gametophytes of oak fern. Background technique [0002] Fern, also known as fern, is an important transitional plant group between bryophytes and seed plants. The main breeding direction of ferns is to cultivate new varieties with strong ornamental value, fast growth and high nutritional value. [0003] Quercus fern is a medium-sized epiphytic fern. Drynaria rhizome is used as medicine in its rhizome, and it is recorded in the "Chinese Pharmacopoeia" as the only genuine drynaria. The accumulation of rhizome biomass of live oak fern under artificial cultivation conditions is very slow, which seriously hinders the research and promotion of the artificial cultivation practice of live oak fern. The biomass accumulation in rhizomes of other species of Quercus genus (such as: Quercus japonica, Sichuan Yunnan Quercus, etc.) is ab...

Claims

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Application Information

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IPC IPC(8): A01G7/04A01H4/00
CPCA01G7/045A01H4/00Y02P60/14
Inventor 石雷黄凤杰李杨李东王頔李静一姚然杨华
Owner INST OF BOTANY CHINESE ACAD OF SCI
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