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Undariapinnatifida seedling cultivation method through parthenogenesis

A parthenogenesis and wakame technology is applied in the field of wakame seedling cultivation using parthenogenesis, and can solve problems such as seedling shedding.

Active Publication Date: 2015-04-15
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, this loose attachment strength causes the seedlings to fall off very seriously when they are temporarily raised at sea after emergence.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The female gametophyte (for the method of obtaining the female gametophyte, please refer to Shan, T.F. and Pang, S.J. 2009. Assessing genetic identity of sporophytic offspring of the brown alga Undaria pinnatifida derived from mono-crossing of gametophyteclones by use of amplified fragment length polymorphismite and microsats Phycol.Res.57:36-44. Recorded in the literature) in PES seawater medium at a temperature of 18°C ​​and a light intensity of 50μmol photons m -2 the s -1 , photoperiod 12h day: 12h night; and after 20 days of static culture under the condition of changing the medium every 3 days, the sporozoites produced by parthenogenesis reached more than 1mm, transferred to a 2L beaker and filled with air, at a temperature of 18°C, Light intensity 50μmol photons m -2 the s -1 , photoperiod 12h daytime: 12h night; and replace the PES seawater medium every 3 days to cultivate until the young sporophytes reach 1cm in length; transfer the young sporophytes with a l...

Embodiment 2

[0028] If large-scale production and cultivation are realized, the parthenogenetically mature sporophytes in Example 1 can be released to produce female gametophytes, and then a large number of parthenogenetically mature spores can be obtained through the above-mentioned induction and the process of parthenogenetic sporophyte life history body.

[0029] When the above-mentioned parthenogenetic sporophytes are mature, cut off the sporophytes, wipe the surface of the sporophytes repeatedly with sterile gauze, remove the miscellaneous algae attached to the surface, dry in the shade for 30-60 minutes, put the sporophylls into the seawater of the nursery pond to release the zoospores, After the seawater color of the released zoospores turns yellowish brown, put them into the seedling curtains (the length of seedling ropes wrapped around each seedling curtain is 100m), and the spore attachment density is controlled at 120 times magnification to 60-100 attached zoospores. The spores ...

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Abstract

The invention relates to an undariapinnatifida cultivation method, in particular to an undariapinnatifida seedling cultivation method through parthenogenesis. The method comprises the steps that induction culture is conducted on female gametophytes until juvenile sporophytes reach more than 1mm, aerated feeding culture is conducted on the female gametophytes, then the life history of parthenogenesis sporophytes is completed, and mature sporophytes are obtained; sporophyls of the sporophytes are placed into sea water to be released to obtain planospores, after the color of the sea water where the planospores are obtained through releasing is changed into yellowish-brown, the planospores are attached to a seedling curtain, and then the seedling curtain is put into fresh sea water; after all the germinal planospores develop into the female gametophytes and are sufficiently grown, light intensity is lowered, the development of the gametophytes is delayed to enable the gametophytes to pull through the high-water-temperature summer; the male gametophytes and the female gametophytes attached to the seedling curtain are simultaneously cultured, and the development and fertilization processes are completed; after reaching about 200 micrometers, the juvenile sporophytes are moved to sea to be cultivated, and then undariapinnatifida cultivation is achieved. According to the undariapinnatifida seedling cultivation method through parthenogenesis, female gametophyte clonal update and enlarged cultivation can be achieved through the parthenogernesis life history, and it is of great significance in clonal preservation and crossbreeding.

Description

technical field [0001] The invention relates to a method for cultivating wakame, in particular to a method for cultivating undaria seedlings by using parthenogenesis. Background technique [0002] There are two ways to cultivate undaria pinnatifida seedlings: zoospore seedlings and gametophyte seedlings. At present, the former is the main method in large-scale seedling production, and the latter only plays an auxiliary role. The main disadvantage of gametophyte seedling collection is that after the gametophyte clone is inoculated to the seedling curtain, it is only attached to the seedling rope by the viscous substance secreted by the algal body, and its firmness is very poor. And the young sporophyte depends on the female gametophyte to attach to the seedling rope before rhizoid formation. Therefore, this loose attachment strength causes the situation of seedling loss to be very serious when the seedlings are temporarily raised at sea after emergence. In contrast, the att...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 单体锋逄少军高素芹
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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