Method for crossbreeding of undaria pinnatifida by using unisexual planospores

A hybrid breeding and zoospore technology, applied in the fields of botanical equipment and methods, application, horticulture, etc., can solve the problem of gametophyte culture expansion that takes a lot of time, manpower and financial resources, the quantity cannot meet production needs in a short time, and the amount of zoospores released. big problem

Active Publication Date: 2014-10-15
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the use of gametophyte seedlings cannot meet the production demand in a short period of time in terms of quantity, and it takes a lot of time, manpower and financial resources to cultivate and expand gametophytes, and the release of zoospores is large, so there is no such drawback

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The female gametophytes and hermaphrodite gametophytes were placed in PES seawater medium at a temperature of 18°C ​​and a light intensity of 60 μmol photons m -2 the s -1 , photoperiod 12h daytime: 12h night; and after 20 days of static culture under the condition of changing the medium every 3 days, the sporophytes reached more than 1mm, transferred to a 2L beaker and filled with air, at a temperature of 18°C ​​and a light intensity of 60μmol photons m -2 the s -1 , photoperiod 12h daytime: 12h night; and replace the PES seawater medium every 3 days to cultivate until the length of the young sporophytes reaches 1cm; transfer the young sporophytes with a length of 1cm to 80 liters of PP water tanks and fill them with natural seawater Air tumbling culture (for tumbling culture methods, please refer to Pang, S.J., Liu, F., Shan, T.F., Gao, S.Q. and Zhang, Z.H. 2009. Cultivation of the brown alga Sargassum horneri: sexual reproduction and seedling production in tank cult...

Embodiment 2

[0033] If large-scale production and cultivation are realized, the sporophytes produced by parthenogenesis and the sporophytes produced by hermaphroditic selfing can be used as seedlings for picking seedlings in production.

[0034] When the above sporophytes are mature, cut off the sporophytes, wipe the surface of the sporophytes repeatedly with sterile gauze, remove the algae attached to the surface, dry in the shade for 30-60 minutes, put the sporophylls into the seawater of the nursery pond to release the zoospores, and wait for release to obtain After the seawater color of the spores turns yellowish brown, put them into the seedling curtain (the length of the seedling rope wrapped on each seedling curtain is 100m), and the spore attachment density is controlled at 120 times magnification. , and then put the seedling curtain into fresh sea water. At a light intensity of 50 μmolphotons m -2 the s -1 , water temperature 18-22°C, photoperiod 12h daytime: 12h night, cultured...

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Abstract

The invention relates to a method for crossbreeding of undaria pinnatifida, in particular to a method for crossbreeding of the undaria pinnatifida by using unisexual planospores. Female gametophytes are subjected to parthenogernesis induction to generate sporophores, meanwhile, hermaphroditic gametophytes are subjected to development cultivation to generate selfing sporophores, when the sporophores are longer than 1mm, the sporophores are changed into inflating cultivation conditions and cultivated to sexual maturity. Mature sporophyls are placed in sea water for releasing the planospores, the planospores are attached on a seeding curtain after the color of sea water turns into tawny, and the seeding curtain is then placed in fresh sea water. The planospores released by the sporophores generated by parthenogernesis are female planospores, and the planospores released by the hermaphroditic gametophytes are male planospores. According to the method, the male and female gametophyte hybridization technique is improved by the male and female planospore hybridization technique, and the method has great significance in applying monoclonal crossbreeding to the production practice in large scales.

Description

technical field [0001] The invention relates to a method for cross-breeding of Undaria pinnatifida, in particular to a method for cross-breeding of Undaria pinnata by using parthenozoites. Background technique [0002] There are two ways to cultivate undaria pinnatifida seedlings: zoospore seedlings and gametophyte seedlings. At present, the former is the main method in large-scale seedling production, and the latter only plays an auxiliary role. The advantage of gametophyte cloning hybrid breeding is that it can produce sporophyte offspring with highly consistent traits, while traditional zoospore breeding will cause the differentiation of sporophyte offspring. But the main shortcoming of gametophyte seedling collection is that after gametophyte clone is inoculated to the seedling curtain, only the viscous substance secreted by the algal body is attached to the seedling rope, and its firmness is very poor. And the young sporophyte depends on the female gametophyte to attac...

Claims

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Application Information

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IPC IPC(8): A01G33/00
Inventor 逄少军李静单体锋
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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