Method for cross-breeding wakame by using parthenozoites
A technology of hybrid breeding and zoospores, applied in botany equipment and methods, applications, horticulture, etc., can solve the problem of time-consuming, manpower and financial resources for gametophyte culture and expansion, the number cannot meet the production demand in a short time, and the release of zoospores major issues
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Embodiment 1
[0026] The female gametophytes and hermaphrodite gametophytes were placed in PES seawater medium at a temperature of 18°C and a light intensity of 60 μmol photons m -2 the s -1 , photoperiod 12h daytime: 12h night; and after 20 days of static culture under the condition of changing the medium every 3 days, the sporophytes reached more than 1mm, transferred to a 2L beaker and filled with air, at a temperature of 18°C and a light intensity of 60μmolphotons m -2 the s -1 , photoperiod 12h daytime: 12h night; and replace the PES seawater medium every 3 days to cultivate until the length of the young sporophytes reaches 1cm; transfer the young sporophytes with a length of 1cm to 80 liters of PP water tanks and fill them with natural seawater Air tumbling culture (please refer to Pang, S.J., Liu, F., Shan, T.F., Gao, S.Q. and Zhang, Z.H. 2009. Cultivation of the brown alga Sargassum horneri: sexual reproduction and seedling production in tank culture under reduced solarirradian...
Embodiment 2
[0033] If large-scale production and cultivation are realized, the sporophytes produced by parthenogenesis and the sporophytes produced by hermaphroditic selfing can be used as seedlings for picking seedlings in production.
[0034] When the above sporophytes are mature, cut off the sporophytes, wipe the surface of the sporophytes repeatedly with sterile gauze, remove the algae attached to the surface, dry in the shade for 30-60 minutes, put the sporophylls into the seawater of the nursery pond to release the zoospores, and wait for release to obtain After the seawater color of the spores turns yellowish brown, put them into the seedling curtain (the length of the seedling rope wrapped on each seedling curtain is 100m), and the spore attachment density is controlled at 120 times magnification. , and then put the seedling curtain into fresh sea water. At a light intensity of 50 μmolphotons m -2 the s -1 , water temperature 18-22°C, photoperiod 12h daytime: 12h night, cultured...
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