A kind of kelp gametophyte gender identification method

Abstract

The invention belongs to the field of seaweed molecular genetics, and in particular relates to a method for identifying the sex of kelp gametophytes. It is characterized in that: Laminaria kelp female gametophyte and male gametophyte specific molecular markers are respectively named SJF and SJM, and their nucleotide sequences are respectively SEQ ID NO: 5 and SEQ ID NO: 6. SEQ ID NO: 5 is amplified from the primer Sf sequence of SEQ ID NO: 3; SEQ ID NO: 6 is amplified from the primer Sm sequence of SEQ ID NO: 4. The invention is accomplished by detecting the above-mentioned specific fragment; the steps include extraction of the Laminaria gametophyte genome DNA to be detected, PCR amplification, and agarose gel electrophoresis detection of the amplification product. In summary, the present invention can clearly identify whether the Laminaria gametophyte is pure female, pure male or mixed sex, which makes up for the deficiencies of the prior art.

Description

technical field [0001] The invention belongs to the field of seaweed molecular genetics, and in particular relates to a method for identifying the sex of kelp gametophytes. Background technique [0002] Kelp (Saccharina japonica) belongs to Phaeophyta (Phaeophyta), Phaeophyceae (Phaeophyceae), Laminariales (Laminariales), Laminariaceae (Laminariaceae), a large brown algae of the genus Saccharina, used in medicine, chemical industry, food and other fields widely. The life history of kelp is divided into two distinct generations, which consist of diploid sporophytes and haploid gametophytes. The sporophytes and gametophytes appear alternately, which is a typical heterogeneous alternation of generations. Fang Zongxi and others first proposed that by separating single gametophyte cells of female and male, under continuous light conditions, only vegetative growth can be carried out to grow into filaments, that is, asexual reproduction line (also called gametophyte cloning line),...

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Problems solved by technology

[0005] Existing problems: the patent document CN106834469A can achieve specific amplification in the male gametophyte of Laminaria, but if the male gametophyte is mixed with the female gametophyte, it cannot be identified

In the same way, the patent document CN102660647A also has the same problem, and the applicant used the marks in the patent to carry out gender identification on the materials of multiple varieties (lines) preserved, and the results showed that it did not have a wide range of specificity; the patent document The female and male markers in CN101280340B are sexed in the materials of multiple varieties (lines) kept in the applicant's laboratory, and the results also show that there are great limitations in specificity

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