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Method for constructing permanent F2 sporophyte population in undaria pinnatifida

A sporophyte and wakame technology, applied in the field of genetic breeding, can solve the problems of underutilization

Active Publication Date: 2021-06-08
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Method for constructing permanent F2 sporophyte population in undaria pinnatifida
  • Method for constructing permanent F2 sporophyte population in undaria pinnatifida
  • Method for constructing permanent F2 sporophyte population in undaria pinnatifida

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Embodiment 1

[0038] Such as figure 1 As shown, utilize parthenogenesis and hermaphroditic gametophyte selfing (acquisition method can obtain according to record in this patent document) mode and obtain homozygous female, male parent sporophyte (DH) respectively (referring to figure 2 ). The length, width and other morphological characteristics of the two are significantly different, and the genetic analysis of 10 SSR loci shows that they have different alleles in 8 loci (80% difference), indicating that the genotype differences between the two are obvious (See Table 1), suitable as parent for population construction. After it matures, isolate female and male gametophytic clones (F 1 Gametophyte) (for how to obtain gametophyte, please refer to Shan, T.F. and Pang, S.J. 2009. Assessing genetic identity of sporophytic offspring of the brown alga Undaria pinnatifida derived from mono-crossing of gametophyte clones by use of amplified fragment length polymorphism and microsatellite markers ...

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Abstract

The invention relates to genetic breeding, in particular to a method for constructing a permanent F2 sporophyte population in undaria pinnatifida, and further provides a method for obtaining a undaria pinnatifida permanent F2 sporophyte population by utilizing full-sib F2 gametophyte clone pairwise hybridization. The method comprises the following steps: respectively separating male and female gametophyte clones (F1 gametophyte) from homozygous parent sporophytes, hybridizing the obtained male and female gametophyte clones to generate F1 sporophytes, selecting a strain of released zoospores after the F1 sporophytes are mature, and performing separating to obtain a large number of F2 gametophyte clones; and randomly selecting female gametophyte clones and male gametophyte clones with the same number from the F2 gametophyte clones, and then completing hybridization of the F2 male gametophyte clones and the F2 male gametophyte clones on the randomly selected gametophytes in a female-male random one-to-one pairing mode, so as to obtain a permanent F2 sporophyte population. The permanent F2 population constructed by the invention has important significance in development of basic genetic research, new variety cultivation and the like in important economic brown seaweed undaria pinnatifida.

Description

technical field [0001] The present invention relates to genetic breeding, specifically a kind of constructing permanent F in wakame 2 The method of sporophyte populations, furthermore, is a method that utilizes full-sib F 2 Permanent F 2 Methods for sporophyte populations. Background technique [0002] Wakame is an important economic brown algae in my country. It belongs to the order Laminaria and has a typical life history of brown algae of the order Laminaria. Most of the important agronomic traits of Wakame are quantitative traits, and the genetic basis of quantitative traits needs to be analyzed to carry out genetic breeding research. The construction of mapping populations is the prerequisite for quantitative trait genetic research, but the current mapping populations in Wakame are basically temporary common F 2 It is difficult to carry out repeated studies at different times and places, and it is impossible to assess the impact of environmental factors on quantita...

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 单体锋逄少军王雪妹李静李倩茜
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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