Method for preventing and controlling benthic diatom pollution in the production of wakame gamete seedlings

A benthic diatom and gametophyte technology, applied in the fields of botanical equipment and methods, seaweed cultivation, application, etc., can solve the problems of sparse seedlings, affecting the quality of seedlings, and the failure of gametophyte seedlings, so as to reduce the shedding rate and save money. Energy cost and labor cost, the effect of avoiding seedling shedding

Active Publication Date: 2022-07-05
DALIAN HAIBAO FISHERY
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Problems solved by technology

However, during this period of time, benthic diatoms will attach and multiply on the seed curtains, compete with the gametophytes and young sporophytes of Undaria pinnatifida for nutrients and growth space, and cover or even cover the gametophytes and young sporophytes, causing the seedlings to be exposed to light. Insufficient and sparse seedlings will affect the quality of seedlings and even lead to the failure of gametophyte seedlings

Method used

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  • Method for preventing and controlling benthic diatom pollution in the production of wakame gamete seedlings
  • Method for preventing and controlling benthic diatom pollution in the production of wakame gamete seedlings
  • Method for preventing and controlling benthic diatom pollution in the production of wakame gamete seedlings

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Experimental program
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Effect test

Embodiment

[0043] Production of wakame gametophyte seed 136 curtains (each curtain seedling rope 37m).

[0044] 1. Gametophyte predevelopment.

[0045] 1.1 Preparation of culture medium: 10L of sterilized seawater boiled for 5 minutes was fired in an electric ceramic furnace using a glass Erlenmeyer flask. Cool to 20°C, add nutrient salt NaNO 3 and KH 2 PO 4 , formulated as NaNO 3 6mg / L, KH 2 PO 4 1mg / L;

[0046] 1.2 Gametophyte crushing and sedimentation: After converting 120g of male and female gametophyte filaments of Wakame (male and female weight ratio 1:1) from the growth state to the development state, filter them with a 350-mesh sieve, add low-temperature sterilized seawater, and use a crusher at 15 The gametocytes were broken into small pieces of 1-6 cells below ℃, and left at 8 ℃ overnight;

[0047] 1.3 Gametophyte pre-development inoculation: the next day, after pouring out the supernatant, inoculate the fragmented male and female mixed gametophyte fragments into 2 Erl...

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Abstract

The invention discloses a method for preventing and controlling benthic diatom pollution in the production of wakame gametophyte seedlings. In the invention, the male and female gametophytes before the pond in the production of wakame gametophyte seedlings are pre-developed and broken, and then they are attached to the pond, so that the wakame gametophyte and juvenile sporophyte compete with the benthic diatoms on the seedling curtain. Occupy an advantage to prevent benthic diatom pollution in the production of wakame gamete seedlings. The technology of the invention has good effect of preventing and controlling benthic diatoms, is convenient and applicable, and can also shorten the cultivation time of the seedlings in the nursery pond and save the production cost.

Description

technical field [0001] The invention relates to the technical field of production of wakame seedlings, in particular to a method for preventing and controlling benthic diatom pollution in the production of wakame gamete seedlings. Background technique [0002] Wakame is one of the three major economic seaweeds in my country. At present, the basic process of conventional wakame seed production process is: algae storage, algae release zoospores, the zoospores adhere to the seedling curtain and develop into embryos, which in turn develop into gametophytes, which release male and female reproductive cells, and fertilize to form juveniles. Sporophyte, grow into wakame seedlings, out of the warehouse. In this production process, because the zoospores adhere firmly to the seedling curtain, the method of flushing the seedling curtain can be used to prevent the contamination of benthic diatoms on the seedling curtain. Less affected. However, conventional seedlings have shortcoming...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00C05G1/00
CPCA01G33/00C05B7/00C05C5/02Y02A40/80
Inventor 张喜昌赵宁宁冷晓飞刘剑波许淑芬
Owner DALIAN HAIBAO FISHERY
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