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Method for constructing double haploid (DH) sporophyte population in undaria pinnatifida

A technology of sporophyte and wakame, applied in the field of genetics and breeding, can solve problems such as no DH population report yet

Active Publication Date: 2021-04-23
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The DH population is widely used in the genetics and genetic breeding research of higher plants. Due to the differences in the life history of various seaweed species, although it has also been established and applied in the economic red alga Porphyra, there has been no report on the DH population in brown algae.

Method used

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  • Method for constructing double haploid (DH) sporophyte population in undaria pinnatifida
  • Method for constructing double haploid (DH) sporophyte population in undaria pinnatifida
  • Method for constructing double haploid (DH) sporophyte population in undaria pinnatifida

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Embodiment 1

[0040] Such as figure 1 As shown, select the F with parthenogenetic ability 1 Cloning of female gametophytes and F 1 Male gametophytic clones, genetic relationship analysis of 10 SSR loci of the selected gametophytic clones showed that female and male gametophytic clones had different alleles at 8 loci (80% difference), indicating that the two The genotype differences are obvious, and they are suitable as parents for population construction. Mix the female and male gametophyte clones at a mass ratio of 1:1, and hybridize after mixing to obtain F 1 Hybrid sporophyte; the hybridization process is the mixing of female and male gametophyte clones in PES seawater medium at a temperature of 18°C ​​and a light intensity of 50μmol photons m -2 the s -1 , photoperiod 12h during the day: 12h at night; and under the condition of changing the medium every 3 days, it was cultured statically for 30 days, and the F produced 1 When the sporophytes reach more than 1mm, they are transferre...

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Abstract

The invention relates to genetic breeding, in particular to a method for constructing a double haploid (DH) sporophyte population in undaria pinnatifida. The invention still further relates to the method for obtaining an undaria pinnatifida DH sporophyte population by utilizing parthenogenesis and hermaphrodite gametophyte selfing. The method comprises the following steps of selecting female gametophytes with parthenogenesis capability for cloning and male gametophytes capable of generating a hermaphroditism phenomenon for cloning, hybridizing the female gametophytes and the male gametophytes to generate F1 sporophytes, selecting a strain to release zoospores after the F1 sporophytes are mature, and separating to obtain a large number of F2 gametophytes for cloning; and selecting the female gametophytes for cloning, obtaining a female DH sporophyte strain by inducing parthenogenesis, selecting the male gametophytes for cloning, and obtaining a male DH sporophyte strain by inducing hermaphroditic phenomenon and selfing, and thereby obtaining the DH sporophyte population. The DH population constructed by the method has important significance in developing basic genetics research, new variety cultivation and the like in important economic brown algae undaria pinnatifida.

Description

technical field [0001] The invention relates to genetic breeding, specifically a method for constructing a DH sporophyte population in Undaria pinnatifida, and further a method for obtaining a DH sporophyte population of Undaria pinnatifida by using parthenogenesis and selfing of hermaphroditic gametophytes. Background technique [0002] Wakame is an important economic brown algae in my country. It belongs to the order Laminaria and has a typical life history of brown algae of the order Laminaria. Most of the important agronomic traits of Wakame are quantitative traits, and the genetic basis of quantitative traits needs to be analyzed to carry out genetic breeding research. The construction of mapping populations is one of the prerequisites for quantitative trait genetic research, but currently the mapping populations in Wakame and even the entire Laminaria order brown algae are basically temporary common F 2 Groups cannot be preserved for a long time, it is difficult to c...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H4/00
CPCA01H1/02A01H1/04A01H4/001A01H4/008Y02A40/80
Inventor 单体锋逄少军王雪妹李静
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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