SCAR molecular marker capable of identifying sea-tangle female gametophyte

Abstract

The invention belongs to the technology field of gene engineering, in particular relates to a sequence characterized amplified regions molecule marker which can distinguish laminaria female gametophyte. The sequence of the molecule marker is SEQ ID No.4. The molecule marker is obtained through the following method: a primer is designed according to an earth wire Y chromosome conservative region and polymerase chain reaction amplification is performed to the laminaria female and female gametophyte gene groups; the original primer is extended to be a novel primer SEQ ID No.3 according to the sequencing result of the amplification product; a sequence characterized amplified regions molecule marker F-494 of the laminaria female gametophyte is obtained through utilizing the primer to perform polymerase chain reaction amplification to the laminaria female and female gametophyte gene groups and the sequence thereof is SEQ ID No.4. The gender of the laminaria gametophyte can be identified rapidly and efficiently through utilizing the sequence characterized amplified regions molecule marker, and the parthenogenesis laminaria sporophyte parental source and the gender of the offspring thereof can be identified.

Description

A SCAR molecular marker that can distinguish female gametophytes in Laminaria technical field The invention belongs to the technical field of genetic engineering, and in particular relates to a specific molecular marker for plant sex identification, in particular to a SCAR molecular marker for distinguishing Laminaria female gametophytes. Background technique For most plants, gender identification is mainly carried out through the external morphology, physiological and biochemical differences, isoenzyme map, specific protein, karyotype, nucleic acid and other aspects of the plant. With the development of molecular biotechnology, especially the maturity of molecular marker technology, the role of molecular markers in plant sex identification is becoming more and more important. Because molecular markers can directly reflect plant differences at the level of genomic DNA, the determination process is not affected by plant developmental stages, which greatly improves economic ...

AI Technical Summary

Problems solved by technology

At present, there are no reports on SCAR molecular markers for identifying female gametophytes of Laminaria at home and abroad

Images