Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-efficiency preparation method for glossy ganoderma polysaccharide

A high-efficiency technology of Ganoderma lucidum polysaccharide is applied in the field of high-efficiency preparation of Ganoderma lucidum crude polysaccharide, which can solve the problems of low polysaccharide purity, low extraction yield, long production cycle, etc., and achieve the effects of improving extraction yield, simple equipment and small energy loss.

Active Publication Date: 2009-02-18
NANJING AGRICULTURAL UNIVERSITY
View PDF1 Cites 28 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a preparation method of Ganoderma lucidum polysaccharide, which overcomes the shortcomings of traditional extraction techniques such as long production cycle, low polysaccharide purity and low extraction yield

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-efficiency preparation method for glossy ganoderma polysaccharide
  • High-efficiency preparation method for glossy ganoderma polysaccharide
  • High-efficiency preparation method for glossy ganoderma polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] (1) Experimental design

[0019] 1. Hot water extraction method (comparison)

[0020] Select water-material ratio, leaching times, leaching temperature and leaching time to do single factor test; orthogonal experiment choose leaching temperature, water-material ratio and leaching time as examination factors, take polysaccharide yield as measuring index, L 9 (3 4 ) Orthogonal test method (Table 1) to determine the optimal process parameters of Ganoderma lucidum polysaccharide hot water extraction.

[0021] 2. Ultrasonic extraction method (scheme of the present invention)

[0022] Ultrasonic power, ultrasonic action time, and water-material ratio were selected as single factor experiments; Box-Benhnken design selected ultrasonic power, ultrasonic action time, and water-material ratio as the examination factors, and polysaccharide yield was used as the measurement index, and the response surface design method was adopted (Table 1). 2), to determine the optimal process p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a preparation method of ganoderma lucidum polysaccharide, which belongs to the field of biological medicine. The fruiting body of ganoderma lucidum is baked and ground until ganoderma lucidum powder is produced; the ground ganoderma lucidum powder is uniformly mixed with pure water which is twenty eight times more than the ganoderma lucidum powder, and smashed by a 680W ultrasonic device for seventeen minutes; after extraction, filtration and dreg removing, the extract is rotationally evaporated until one quarter of the original volume; 95 percent ethanol, the volume of which is four times larger than the volume of the concentrated solution, is slowly added into the concentrated solution; after twelve hours of ethanol precipitation under a temperature equal to 4 DEG C and fifteen minutes of centrifugation at 4500rpm, supernatant is removed; and after being washed respectively by absolute ethyl alcohol, acetone and ether, precipitate is baked in an oven under a temperature equal to 55 DEG C in order to obtain the crude ganoderma lucidum polysaccharide. Since the ultrasonic extraction technique is adopted, the yield can reach 20.79 plus or minus 0.11mg / g, purity can reach 47.88 percent, and compared with the conventional hot water soaking extraction, the time is shorten by 96 percent while the yield is increased by 28 percent. The obtained ganoderma lucidum polysaccharide can be widely used as the base of drugs and health-caring food.

Description

1. Technical field [0001] The invention relates to a high-efficiency preparation method of Ganoderma lucidum crude polysaccharide, which belongs to the field of biomedicine, and the product can be widely used in the fields of health care products, functional foods, biomedicine and the like. 2. Background technology [0002] Ganoderma lucidum is a large basidiomycete with high nutritional and health value. Since ancient times, Ganoderma lucidum has been regarded as a symbol of auspiciousness, wealth, beauty and longevity. So far, we have isolated more than 150 compounds from Ganoderma lucidum, which can be divided into polysaccharides, triterpenes, nucleosides, furans, alkaloids, amino acids, proteins, sterols, inorganic ions, organic germanium, etc. More than a dozen types of substances, among which polysaccharides have the functions of anti-tumor growth, reducing the side effects of chemotherapy, calming the mind, improving the body's immunity, strengthening the heart, low...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00A61P37/02A61P35/00A61P3/10A61P3/06A61P7/02A61P39/06
Inventor 胡秋辉董艳红杨方美赵立艳安辛欣辛志宏郑惠华
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com