High-efficiency preparation method for glossy ganoderma polysaccharide
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A high-efficiency technology of Ganoderma lucidum polysaccharide is applied in the field of high-efficiency preparation of Ganoderma lucidum crude polysaccharide, which can solve the problems of low polysaccharide purity, low extraction yield, long production cycle, etc., and achieve the effects of improving extraction yield, simple equipment and small energy loss.
Active Publication Date: 2009-02-18
NANJING AGRICULTURAL UNIVERSITY
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[0006] The purpose of the present invention is to provide a preparation method of Ganoderma lucidum polysaccharide, which overcomes t
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[0018] (1) Experimental design
[0019] 1. Hot water extraction method (comparison)
[0020] Select water-material ratio, leaching times, leaching temperature and leaching time to do single factor test; orthogonal experiment choose leaching temperature, water-material ratio and leaching time as examination factors, take polysaccharide yield as measuring index, L 9 (3 4 ) Orthogonal test method (Table 1) to determine the optimal process parameters of Ganoderma lucidum polysaccharide hot water extraction.
[0021] 2. Ultrasonic extraction method (scheme of the present invention)
[0022] Ultrasonic power, ultrasonic action time, and water-material ratio were selected as single factor experiments; Box-Benhnken design selected ultrasonic power, ultrasonic action time, and water-material ratio as the examination factors, and polysaccharide yield was used as the measurement index, and the response surface design method was adopted (Table 1). 2), to determine the optimal process p...
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Abstract
The present invention discloses a preparation method of ganoderma lucidum polysaccharide, which belongs to the field of biological medicine. The fruiting body of ganoderma lucidum is baked and ground until ganoderma lucidum powder is produced; the ground ganoderma lucidum powder is uniformly mixed with pure water which is twenty eight times more than the ganoderma lucidum powder, and smashed by a 680W ultrasonic device for seventeen minutes; after extraction, filtration and dreg removing, the extract is rotationally evaporated until one quarter of the original volume; 95 percent ethanol, the volume of which is four times larger than the volume of the concentrated solution, is slowly added into the concentrated solution; after twelve hours of ethanol precipitation under a temperature equal to 4 DEG C and fifteen minutes of centrifugation at 4500rpm, supernatant is removed; and after being washed respectively by absolute ethyl alcohol, acetone and ether, precipitate is baked in an oven under a temperature equal to 55 DEG C in order to obtain the crude ganoderma lucidum polysaccharide. Since the ultrasonic extraction technique is adopted, the yield can reach 20.79 plus or minus 0.11mg/g, purity can reach 47.88 percent, and compared with the conventional hot water soaking extraction, the time is shorten by 96 percent while the yield is increased by 28 percent. The obtained ganoderma lucidum polysaccharide can be widely used as the base of drugs and health-caring food.
Description
1. Technical field [0001] The invention relates to a high-efficiency preparation method of Ganoderma lucidum crude polysaccharide, which belongs to the field of biomedicine, and the product can be widely used in the fields of health care products, functional foods, biomedicine and the like. 2. Background technology [0002] Ganoderma lucidum is a large basidiomycete with high nutritional and health value. Since ancient times, Ganoderma lucidum has been regarded as a symbol of auspiciousness, wealth, beauty and longevity. So far, we have isolated more than 150 compounds from Ganoderma lucidum, which can be divided into polysaccharides, triterpenes, nucleosides, furans, alkaloids, amino acids, proteins, sterols, inorganic ions, organic germanium, etc. More than a dozen types of substances, among which polysaccharides have the functions of anti-tumor growth, reducing the side effects of chemotherapy, calming the mind, improving the body's immunity, strengthening the heart, low...
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