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Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus

A technology for comprehensive extraction of Inonotus obliquus, which is applied in the field of polysaccharide and polyphenol extraction and steroid compounds, can solve the problems of low polyphenol yield, environmental pollution, and unseen problems, and achieve the promotion of comprehensive utilization and recovery The effect of high efficiency and enhanced activity

Active Publication Date: 2012-07-18
陈国全
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the method of water extraction, ethanol precipitation or solvent extraction is usually used for the extraction and separation of polysaccharides and polyphenols in Inonotus obliquus. Due to the low yield of conventional water extraction and ethanol precipitation extraction methods, the yield of polysaccharides in Inonotus obliquus is only 2 %~5%, the polyphenol yield of Inonotus obliquus is lower, and the solvent extraction method will cause environmental pollution due to the use of a large amount of organic solvents
And have not seen a kind of report and the example of the method that can simultaneously extract steroid compound, polysaccharide and polyphenol in Inonotus obliquus so far

Method used

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  • Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus
  • Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus
  • Method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Inonotus obliquus is pulverized into powder in an electric mill, and then subjected to high pressure treatment in an autoclave (100KPA, 121°C) for 1 hour to pre-treat the raw materials;

[0028] 2) adding the above-mentioned high-pressure Inonotus obliquus into the ethanol of 80% mass concentration, the mass ratio of the Inonotus obliquus to ethanol is 1:20, and then reflux extraction at a temperature of 90 ° C for 2 hours, Repeat the leaching 3 times, then filter under reduced pressure, and combine the filtrates to obtain the test solution of Inonotus obliquus containing steroids;

[0029] 3) under the temperature of 60 ℃ and the pressure of 0.1MPa, the above-mentioned Inonotus obliquus test solution containing steroids is placed in a rotary evaporator under reduced pressure and rotary-evaporated to no ethanol, and the volume is the above-mentioned concentrated Chaga. Suspended and washed with 10 times the amount of pore bacteria test solution in water, and then cen...

Embodiment 2

[0037] 1) Grind Inonotus obliquus into powder in an electric mill, and then high-pressure treatment in an autoclave (100KPA, 121°C) for 2 hours to pre-treat the raw materials;

[0038]2) adding the above-mentioned high-pressure Inonotus obliquus to the ethanol of 80% mass concentration, the mass ratio of the Inonotus obliquus to ethanol is 1:15, and then reflux extraction at a temperature of 80 ° C for 1.5 hours, Repeat the leaching 3 times, then filter under reduced pressure, and combine the filtrates to obtain the test solution of Inonotus obliquus containing steroids;

[0039] 3) Under the temperature of 50 ℃ and the pressure of 0.08MPa, the above-mentioned Inonotus obliquus test solution containing steroids is placed in a rotary evaporator under reduced pressure and rotary-evaporated to no ethanol, and the volume is the above-mentioned concentrated Chaga. The pore bacteria test solution was washed by suspending and washing with 7 times the amount of water, and then centrif...

Embodiment 3

[0047] 1) Grind Inonotus obliquus into powder in an electric mill, and then high-pressure treatment in an autoclave (100KPA, 121°C) for 3 hours to pre-treat the raw materials;

[0048] 2) adding the above-mentioned high-pressure-treated Inonotus obliquus into the ethanol of 80% mass concentration, the mass ratio of the inonotus obliquus to the ethanol is 1:10, and then refluxing and extracting at a temperature of 80 ° C for 1 hour, Repeat the leaching 3 times, then filter under reduced pressure, and combine the filtrates to obtain the test solution of Inonotus obliquus containing steroids;

[0049] 3) Under the temperature of 40 ℃ and the pressure of 0.06MPa, the above-mentioned Inonotus obliquus test solution containing steroids is placed in a rotary evaporator under reduced pressure and rotary-evaporated to no ethanol, and the volume is the above-mentioned concentrated Chaga. The pore bacteria test solution was washed by 5 times of water suspension, and then centrifuged to c...

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Abstract

The invention relates to a method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus. The method mainly comprises the following steps: high-pressure treatment, ethanol extraction, concentration, centrifugation, deionized water extraction, concentration, ethanol precipitation, separation, purification, freezing and drying. Compared with the conventional extraction method, the provided method has the biggest difference in that high-pressure treatment is carried out to the raw material for preliminary treatment under the appropriate time condition, then an extract containing steroids is obtained by an ethanol extraction method, the steroidal compounds are purified by a water washing method and a centrifugation method, extracts of polysaccharides and polyphenols are obtained by a water extraction method, and then the polysaccharides and polyphenols are separated through ethanol precipitation. The tissue structure of inonotus obliquus changes due to high-pressure treatment, therefore, the yield of the steroidal compounds, polysaccharides and polyphenols is high, the molecular structure of active components in inonotus obliquus changes under high pressure, and the study verifies that the activity of inonotus obliquus can be enhanced.

Description

technical field [0001] The invention relates to a method for extracting steroids, polysaccharides and polyphenols, in particular to a method for comprehensively extracting steroids, polysaccharides and polyphenols from Inonotus obliquus. Background technique [0002] Inonotus obliquus belongs to the fungal phylum, Basidiomycota, Stratamycetes, Non-Brown Fungi, Phaeoporousobliquus (per: Fr.) J.Schroet. At present, the chemical composition of Inonotus obliquus mainly includes lanolin steroids, polysaccharides, polyphenols, lignin, melanin, alkaloids, tannins, folic acid derivatives and aromatic vanillic acid, Syringic acid and γ-hydroxybenzoic acid, etc. At present, relevant researchers have carried out research on the biological activities of these components and applied them in the prevention and treatment of malignant tumors, diabetes, cardiovascular diseases, liver diseases and AIDS. [0003] Steroids are one of the main active ingredients in Inonotus obliquus, with larg...

Claims

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Application Information

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IPC IPC(8): A61K36/07C08B37/00A61P37/02A61P35/00A61P31/18A61P9/00A61P3/10A61P1/16
Inventor 陈海霞马利帅张宁章钰
Owner 陈国全
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