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Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients

A technology of Inonotus obliquus and Inonotus obliquus, which is applied to the effective hypoglycemic part of Inonotus obliquus and its preparation and application field, can solve the problems of enrichment and separation of active substances, reduce adverse side effects, and have market competitiveness Advantages, the effect of reducing the toxicity of clinical medication

Inactive Publication Date: 2011-05-04
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Judging from the current situation and industry of developing Inonotus obliquus at home and abroad, the activity of Inonotus obliquus is generally recognized, but they are all crude extracts based on decoction or water extraction of fruiting bodies, and no active substances have been tested. Enrichment and separation, and the development of active components into new and efficient health products or medicines

Method used

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  • Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients
  • Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients
  • Fuscoporia obliqua active ingredients capable of lowering blood sugar and preparation method and application of fuscoporia obliqua active ingredients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1, the extraction and separation of the active polysaccharide of Inonotus obliquus fruiting bodies

[0021] (1) After pulverizing the fruiting bodies of Inonotus obliquus, pass through a 40-mesh sieve.

[0022] (2) Preparation of crude polysaccharide extracted at normal temperature: add 30 times the volume of water to the pulverized and sieved Inonotus obliquus, let it stand for extraction for 48 hours, centrifuge the extract (4000r / min), leave the residue, collect the supernatant, Concentrate under reduced pressure to 1 / 10 of the original volume, add absolute ethanol until the final concentration of ethanol is 80%, let it stand overnight at 4°C, collect the precipitate by centrifugation, dry and weigh at 50°C, and obtain crude polysaccharide extracted at room temperature. The polysaccharide content was determined by the phenol-sulfuric acid method, and the calculated polysaccharide yield (polysaccharide yield=(crude polysaccharide weight / material weight)×100%) ...

Embodiment 2

[0027] Example 2, Evaluation of hypoglycemic pharmacological activity of each polysaccharide component of Inonotus obliquus

[0028] (1) Test material:

[0029] The crude extract of Inonotus obliquus and each polysaccharide component of Inonotus obliquus were prepared according to the method in Example 1. Positive control drug: metformin hydrochloride tablets, Beijing Pharmaceutical Co., Ltd., specification: 0.25g×50 tablets / box. Streptozotocin (STZ): American sigma company. Glucose oxidase kit: Beijing Jiuqiang Biotechnology Co., Ltd. Triglyceride kit: Beijing Beihua Kangtai Clinical Kit Co., Ltd. Enzymatic Total Cholesterol Determination Kit: Zhongsheng Beikong Biotechnology Co., Ltd. Insulin radioimmunoassay kit: China Institute of Atomic Energy.

[0030] Test animals: SPF grade ICR mice, male, weighing 18-22g. Provided by the Experimental Animal Center of the Academy of Military Sciences. The animal license number is SCXK (Army) 2002-001.

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Abstract

The invention discloses fuscoporia obliqua active ingredients capable of lowering blood sugar and a preparation method and application of the fuscoporia obliqua active ingredients. The preparation method takes fuscoporia obliqua fruit body as raw material and comprises the following steps: respectively extracting, filtering and concentrating the fuscoporia obliqua fruit body with normal temperature water and high temperature water; adding alcohol into concentrate and depositing to obtain crude polysaccharide; respectively pouring the polysaccharide extracted with normal temperature water and the crude polysaccharide extracted with high temperature water to flow through a (diethylaminoethanol) DEAE-52 cellulose column; carrying out subsection elution by using distilled water and NaCl solutions with different concentrations; and collecting stepwise elution peak sugar solution. Internal blood sugar reduction activity experiment shows that 0.2mol / L NaCl-section eluted sugar of the crude polysaccharide extracted with normal temperature water and 0.2mol / L NaCl-section eluted sugar of the crude polysaccharide extracted with high temperature water both have obvious blood sugar reduction activity, same blood sugar reduction activity with the blood sugar reduction medicine of metformin hydrochloride, and no obvious toxic or side effect.

Description

technical field [0001] The invention relates to an effective fraction of Inonotus obliquus for lowering blood sugar, a preparation method and application thereof. Background technique [0002] The scientific name of Inonotus obliquus is Fuscoporia obliqua or Inonotus obliquus, which belongs to the phylum Fungi, Basidiomycotina, Phytomycetes, Non-Brownomycetes, and the genus Brown Sculptorum (Fiberoporus), aliased as Chaga and Chaga Velvet and Inonotus obliquus, born under the bark of birch, elm, alder, etc., or under the bark of living trees or on the dead after felling. Since the 16th and 17th centuries, Eastern Europe, Russia, Poland, Finland, etc. have widely used it to prevent and treat various difficult and miscellaneous diseases, such as various cancers (gastric cancer, liver cancer, intestinal cancer, etc.), heart disease, diabetes, and can inhibit HIV and radiation. Inhibition of protein biosynthesis, anti-mitosis and elimination of its activity is called a miraculo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07C08B37/00A61P3/10A61P3/06
Inventor 刘萍路春桃张莹罗岩
Owner CHINA AGRI UNIV
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