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Method for extracting rice bran polysaccharide from sub-critical water

A technology of rice bran polysaccharide and subcritical water, which is applied in the field of separation of active components of natural products, can solve the problems of low method yield and limited improvement of extraction yield, and achieves the effects of simple operation, short extraction time and good dissolution performance.

Active Publication Date: 2011-05-18
GUANGDONG SHENGYUAN ZHONGTIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yield of water extraction method is low, and the extraction yield of rice bran polysaccharides assisted by ultrasonic or microwave water extraction is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 10g of defatted rice bran powder, put it into the extraction kettle, pump 250mL of water with pH5.0 into the extraction kettle, control the pressure inside the extraction kettle to 5MPa, and the temperature to 140°C. After extraction for 50min, cool down, centrifuge at 3000g / min for 8min, and collect the The supernatant was concentrated under reduced pressure to 1 / 10 of the original volume, added trichloroacetic acid with a final concentration of 8% to remove the protein, repeated the protein removal 3 times, 3500g / min, centrifuged for 10min, and the supernatant was precipitated with ethanol with a final concentration of 70% , let stand at 4°C for 24 hours, collect the precipitate by filtration, and freeze-dry to obtain rice bran polysaccharide powder with an extraction yield of 3.1%.

Embodiment 2

[0022] Take 10g of defatted rice bran powder, put it into the extraction kettle, pump 250mL of water with pH5.0 into the extraction kettle, control the pressure inside the extraction kettle to 8 MPa, and the temperature to 150°C, extract for 30 minutes, cool, centrifuge at 3000g / min for 8 minutes, and collect The supernatant was concentrated under reduced pressure to 1 / 10 of the original volume, added trichloroacetic acid with a final concentration of 9% to remove protein, and repeated protein removal 3 times at 3500g / min, centrifuged for 10min, and the supernatant was added with a final concentration of 70% trichloroacetic acid Precipitate with ethanol, let stand at 4°C for 24 hours, collect the precipitate by filtration, and freeze-dry to obtain rice bran polysaccharide powder with an extraction yield of 3.8%.

Embodiment 3

[0024] Take 10g of defatted rice bran powder, put it into the extraction kettle, pump 250mL of water with pH 5.0 into the extraction kettle, control the pressure inside the extraction kettle to 10 MPa, and the temperature to 160°C. After extraction for 20 minutes, cool down, centrifuge at 3000g / min for 8 minutes, and collect The supernatant was concentrated under reduced pressure to 1 / 10 of the original volume, added trichloroacetic acid with a final concentration of 10% to remove the protein, repeated the protein removal 3 times, 3500g / min, centrifuged for 10min, and the supernatant was added with a final concentration of 80% trichloroacetic acid Precipitate with ethanol, let stand at 4°C for 24 hours, collect the precipitate by filtration, freeze-dry to obtain rice bran polysaccharide powder, and the calculated extraction yield is 4.1%.

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Abstract

The invention discloses a method for extracting rice bran polysaccharide from sub-critical water, belonging to the technical field of separation of active components of natural products. The method comprises the following steps of: crushing the degreased rice bran and sieving with a screen with 60 meshes; feeding the sieved rice bran into an extraction kettle; pumping water into the extraction kettle; controlling the pressure of the extraction kettle to be 5-20MPa, the extraction temperature to be 100-200DEG C and the extraction time to be 5-60 minutes; cooling and filtering; removing protein with trichloroacetic acid; centrifuging and precipitating supernate with ethanol; collecting the precipitation, freezing and drying to obtain rice bran polysaccharide. The rice bran polysaccharide has the advantages of high extraction yield, low energy consumption, cleanness of process, environment friendliness and simple process. The invention is more convenient for researching the structure and functions of the rice bran polysaccharide and developing products with high added value of the rice bran polysaccharide.

Description

technical field [0001] The invention relates to an extraction method of active polysaccharides, in particular to a method for extracting active polysaccharides with subcritical water, and belongs to the technical field of separation of active components of natural products. Background technique [0002] Subcritical water extraction (sub-critical water extraction, SWE) uses water as the extraction solvent. Water is a non-toxic and cheap solvent. Water at normal temperature and pressure is a very polar solvent and can only dissolve polar organic substances. Substance, very little solubility for medium polar and non-polar substances. But by controlling the temperature and pressure of water, the polarity, surface tension and viscosity of water can be changed, and the solubility of organic matter in water can be increased. Under proper pressure, when the water temperature is heated to between 100-374°C, the water still maintains a liquid state, but the polarity of the water cha...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张海晖段玉清任晓锋武研徐菲菲秦宇
Owner GUANGDONG SHENGYUAN ZHONGTIAN BIOTECH CO LTD
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