Enteromorpha polysaccharide having immunocompetence and preparation method thereof

The technology of prolifera polysaccharide and immune activity is applied in the field of prolifera polysaccharide with immune activity and its preparation, which can solve the problems of wasting high-quality resources, affecting the appearance of water bodies, increasing treatment costs and the like, and achieving the effect of good immune activity.

Inactive Publication Date: 2012-04-04
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, Enteromorpha in the Yellow Sea has broken out in a large area, seriously affecting the beauty of the water body and polluting the sea environment
Usually, Enteromorpha is discarded as garbage, causing environmental pollution, increasing processing costs, and wasting high-quality resources

Method used

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  • Enteromorpha polysaccharide having immunocompetence and preparation method thereof
  • Enteromorpha polysaccharide having immunocompetence and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation method of HT-II polysaccharide:

[0024] 1. Extraction: Take a sample of Enteromorpha from the sea area of ​​Qingdao, wash the sample of Enteromorpha with tap water, take 100g after natural air-drying, break it up, add distilled water with the mass ratio of Enteromorpha slag and water as 1:30, and extract at 95°C After 2 hours, filter with suction, and extract the filter residue twice with 1:15 distilled water. The filtrates were combined, concentrated by rotary evaporation until the liquid was slightly viscous, 3 times the volume of 95% ethanol was added, and allowed to stand overnight at 4°C. Centrifuge at 9000rpm, dehydrate the precipitate with 85%, 95%, absolute ethanol and ether in turn, and freeze-dry to obtain the crude polysaccharide HT.

[0025] 2. Deproteinization: make the crude polysaccharide into 5% sugar solution, use papain, take protease into the sugar solution according to the mass ratio of enzyme and crude polysaccharide ...

Embodiment 2

[0027] Embodiment 2: the immune activity test of HT-II polysaccharide:

[0028] 1. The effect of HT-II polysaccharide on the proliferation of spleen lymphocytes: using MTS-PMS colorimetric method. BALB / c mice were sacrificed by breaking the cervical spine, and the spleen was taken aseptically to prepare splenic lymphocytes, and the cell concentration was adjusted to 410 with RPMI1640 complete culture medium 9 / L cell / ml.

[0029] BALB / c mice 410 9 / L cell / ml splenocyte suspension was added to a 96-well cell culture plate, 100 μl per well, and HT diluted with RPMI1640 complete culture medium was added to each well of the polysaccharide group to a concentration of: 6.25, 12.5, 25, 50, 100 mg / L 10 μl each of -II, 5 repetitions for each concentration, and the blank control group was replaced by an equal volume of RPMI1640. Set at 37°C, 5% CO 2 Cultivate for 72 hours in a saturated humidity incubator. Take it out, add 20μl MTS-PMS to continue culturing for 4-6 hours, and measu...

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Abstract

The invention discloses an enteromorpha polysaccharide having immunocompetence and a preparation method thereof. The enteromorpha polysaccharide having immunocompetence is a polysaccharide HT-II. The enteromorpha polysaccharide having immunocompetence is a white floccule, can be well dissolved in water and dimethyl sulfoxide, has relative molecular weight of 96kDa, and mainly comprises glucose and mannose, wherein a ratio of rhamnoside to xylose to mannoside to glucose is 36: 20: 6: 38. The preparation method comprises the following steps of breaking down, carrying out extraction at a temperature of 90 DEG C by water, filtrating, merging the extract, concentrating, carrying out precipitation by ethanol, removing proteins, carrying out separation and purification, and carrying out freeze drying to obtain the purified enteromorpha polysaccharide having immunocompetence. The preparation method is simple, has stable performances, a low cost and a large medical value, can reduce cholesterol content and can improve immunity. The preparation method adopts extraction, protein removal and purification processes, and scientific experimental means, is a novel method for controlling enteromorpha, and provides a novel approach for controlling enteromorpha. The preparation method of the enteromorpha polysaccharide having immunocompetence develops enteromorpha medical values and purposes, controls environmental pollution, reduces a clean-up cost, improves economic benefits and is an ideal special preparation technology for enteromorpha control and integrated utilization.

Description

technical field [0001] The invention relates to medical biotechnology, in particular to an enteromorpha polysaccharide with immune activity extracted from natural seaweed such as enteromorpha and a preparation method thereof. Background technique [0002] As we all know, Enteromorpha is a kind of large green algae. It is a natural wild green algae in the coastal flats. Its natural reproductive ability is particularly strong and its output is huge. The green algae in my country's waters, especially the East China Sea, is mainly Enteromorpha, which is very rich in resources. The annual natural output (fresh weight) of Fujian coastal area alone reaches more than 100,000 tons. In recent years, Enteromorpha in the Yellow Sea has broken out in a large area, seriously affecting the beauty of the water body and polluting the sea environment. Usually, Enteromorpha is discarded as garbage, causing environmental pollution, increasing processing costs, and wasting high-quality resource...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04A61P39/00A23L1/09
Inventor 李江林学政黄晓航谭姣姣
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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