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Process for extracting tea polyphenol, caffeine as a byproduct thereof and tea polysaccharide from tea

A technology of caffeine and tea polyphenols, which is applied in the field of extracting tea polyphenols by-products of caffeine and tea polysaccharides, can solve the problems of toxic solvent residues, etc., and achieve the effects of improving extraction rate, significant effect, and increasing diffusion area

Inactive Publication Date: 2005-11-23
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it uses dichloromethane to elute caffeine, which also brings problems such as toxic solvent residues
In addition, the swelling of the resin by dichloromethane will also cause problems for long-term industrial applications

Method used

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  • Process for extracting tea polyphenol, caffeine as a byproduct thereof and tea polysaccharide from tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1) Add 1kg of tea leaves, add 20L of water, heat up to 40°C, adjust the pH range to 4.5, add 0.8g of compound enzyme solution, and extract for 60 minutes; add 15L of water to the filtered filter residue, temperature 60°C, pH value of 6, and extract for 40 minutes .

[0052] 2) Combine the extracts twice, filter with a polysulfone hollow fiber ultrafiltration membrane with a molecular weight cut-off of 30kD, and precipitate the membrane concentrate to obtain crude tea polysaccharides. Trichloroacetic acid precipitates protein, and the filtrate after filtering protein is subjected to alcohol precipitation, filtration, washing and drying to obtain tea polysaccharide. The sugar content of tea polysaccharide is 50.5%, and the yield accounts for 2.5% of the dry weight of tea leaves.

[0053] 3) The XAD7 resin column on the membrane permeate absorbs tea polyphenols and caffeine. In the resin column after adsorption, the first stage uses 8BV (BV value is the resin bed volume)...

Embodiment 2

[0056] 1) Add 30L of water to 1kg of tea leaves, raise the temperature to 50°C, adjust the pH value to 5.5, add 2.0g of compound enzyme solution, and extract for 70 minutes. Add 10 L of water to the filtered filter residue at a temperature of 50° C. and a pH value of 6, and extract for 30 minutes.

[0057] 2) Combine the extracts twice, filter with a polysulfone hollow fiber ultrafiltration membrane with a molecular weight cut-off of 50kD, the membrane concentrate is precipitated by ethanol to obtain crude tea polysaccharides, and after dissolving the crude polysaccharides in water, add 1 / 10 volume of 5% trichloride Acetic acid precipitation and deproteinization, and the filtered filtrate is subjected to alcohol precipitation, filtration, washing and drying to obtain tea polysaccharide. The sugar content of tea polysaccharide is 53.2%, and the yield accounts for 2.4% of the dry weight of tea leaves.

[0058] 3) HP on membrane permeate 2 The MG resin column adsorbs tea polyph...

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PUM

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Abstract

Disclosed is a process for extracting tea polyphenol, caffeine as a byproduct thereof and tea polysaccharide from tea, which comprises, (1) extracting active ingredients including tea polyphenol, caffeine and tea polysaccharide from the tea through composite enzymolysis abstraction and water abstraction, (2) filtering the raffinate with hyperfiltration membrane, (3) subjecting the membrane compression liquid to ethanol deposition so as to obtain crude polysaccharides, removing protein and carrying out ethanol deposition, drying to obtain the tea polysaccharides, (4) separating and purifying through second level resin adsorption chromatography, thus obtaining tea polyphenol as well as caffeine as by-product.

Description

technical field [0001] The invention relates to a process for extracting tea polyphenols from tea leaves to by-product caffeine and tea polysaccharides. Background technique [0002] Tea polyphenols are natural, multi-functional, high-efficiency, excellent antioxidants and free radical scavengers. The health functions of tea polyphenols mainly include eliminating free radicals in the body, anti-aging and anti-cancer, anti-radiation, antibacterial, and sterilizing, etc. . Caffeine excites the central nervous system, and has physiological and health functions such as strengthening the heart, diuresis, and detoxification, and is an important raw material for medicine, health care products, and beverages. Tea polysaccharide has various pharmacological effects such as lowering blood sugar, lowering blood fat, anticoagulation, antithrombosis, resistance to hypoxia, enhancing body immunity, anti-inflammation, and anti-cancer. Functional natural medicine. [0003] At present, th...

Claims

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Application Information

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IPC IPC(8): C12P17/06
Inventor 张效林
Owner NORTHWEST UNIV
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