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Ganoderan extraction method and ganoderan use

A technology of Ganoderma lucidum polysaccharide and extraction method, which is applied in the directions of tumor/cancer cells, vertebrate cells, animal cells, etc., to achieve the effects of good antioxidant effect, inhibiting the proliferation of A549 cells, and shortening the extraction time.

Active Publication Date: 2013-05-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the inhibitory effect of Ganoderma lucidum polysaccharide on human lung cancer cell A549

Method used

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  • Ganoderan extraction method and ganoderan use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Add 95% ethanol to the crushed Ganoderma lucidum, extract by boiling for 3 hours, filter to obtain Ganoderma lucidum residue, and dry at 40°C to obtain Ganoderma lucidum powder for later use;

[0029] (2) Take 50g of Ganoderma lucidum powder and put it in a container, mix it with deionized water at a ratio of 1:30, use the traditional water extraction method, extract by boiling for 3 hours, and filter to obtain the extract;

[0030] (3) Perform ultrafiltration on the extract obtained in step (2) with a membrane with a molecular weight cut-off of 10,000 Daltons, and collect the permeate;

[0031] (4) Vacuum concentrate the ultrafiltration permeate to 1 / 3 of the original volume to obtain a concentrate;

[0032] (5) Add 95% ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 70% (v / v), let it stand at 4°C for 12 hours, filter, remove the supernatant part, and the polysaccharide precipitates;

[0033] (6) The precipitate was...

Embodiment 2

[0036] (1) Add 95% ethanol to the crushed Ganoderma lucidum, extract by boiling for 3 hours, filter to obtain Ganoderma lucidum residue, and dry at 40°C to obtain Ganoderma lucidum powder for later use;

[0037] (2) Take 50g of Ganoderma lucidum powder and put it in a container, mix it with a citric acid solution with a pH value of 3 at 1:30, add 1.5% (material) fungal acid protease, ultrasonic power is 640W, ultrasonic temperature is 70°C, extract 60 Minutes, filtered to obtain the extract;

[0038] (3) Perform ultrafiltration on the extract obtained in step (2) with a membrane with a molecular weight cut-off of 10,000 Daltons, and collect the permeate;

[0039] (4) Vacuum concentrate the ultrafiltration permeate to 1 / 3 of the original volume to obtain a concentrate;

[0040] (5) Add 95% ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 70% (v / v), let it stand at 4°C for 12 hours, filter, remove the supernatant part, and the pol...

Embodiment 3

[0045] (1) Add 95% ethanol to the crushed Ganoderma lucidum, extract by boiling for 4 hours, filter to obtain Ganoderma lucidum residue, and dry at 60°C to obtain Ganoderma lucidum powder for later use;

[0046] (2) Take 50g of Ganoderma lucidum powder and put it in a container, mix it evenly with citric acid solution with a pH value of 2 at 1:25, add 0.5% (material) fungal acid protease, ultrasonic power is 320W, ultrasonic temperature is 50°C, extract 40 Minutes, filtered to obtain the extract;

[0047] (3) Perform ultrafiltration on the extract obtained in step (2) with a membrane with a molecular weight cut-off of 10,000 Daltons, and collect the permeate;

[0048] (4) Vacuum concentrate the ultrafiltration permeate to 1 / 4 of the original volume to obtain a concentrate;

[0049] (5) Add 95% ethanol to the concentrated solution, after mixing evenly, the final concentration of ethanol is 60% (v / v), let it stand at 0°C for 8 hours, filter, remove the supernatant part, and the...

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Abstract

The invention discloses a ganoderan extraction method and a ganoderan use. The ganoderan extraction method utilizes an ultrasonic enzyme method to realize ganoderan extraction and comprises ganoderma lucidum crushing, extraction, ultrafiltration, condensation and ethanol precipitation separation. The ganoderan extraction method has a ganoderan extraction rate of 3.14 to 4.21%. The ganoderan obtained by the ganoderan extraction method has molecular weight less than 10000 dalton and is light brown. The ganoderan extraction method has simple extraction separation processes and is suitable for industrial production. Ganoderan has strong antioxidant activity and anticancer activity. In a DPPH. free radical system, IC50 of ganoderan is in a range of 0.28 to 0.43mg / mL and an ORAC value of ganoderan is in a range of 1207.66 to 1777.90 micromole Trolox / g. When a concentration of ganoderan is 2mg / ml, ganoderan has a human lung cancer cell A549 inhibition rate of 53.04 to 68.37 and can be used for preparation of an anticancer drug.

Description

technical field [0001] The invention relates to the deep processing technology of ganoderma lucidum, in particular to the extraction method and application of ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma Lucidum is a fungus of the genus Ganoderma in the family Polyporaceae Basidiomycetes, and is a precious medicinal material with a medicinal history of thousands of years. "Shen Nong's Materia Medica" records that Ganoderma lucidum has the functions of strengthening the body, strengthening the body, nourishing and strengthening, and prolonging life. Modern medical research shows that Ganoderma lucidum has various pharmacological effects such as anti-tumor, enhancing immune function, lowering blood sugar, protecting liver, anti-aging, anti-inflammation, and anti-coagulation. As one of the main active components of Ganoderma lucidum, Ganoderma lucidum polysaccharide has gradually attracted the attention of researchers in recent years. For example,...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12N5/09
Inventor 游丽君冯梦莹赵谋明崔春刘钧发任娇艳
Owner SOUTH CHINA UNIV OF TECH
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