Method for preparing bioflocculation by Bacillus licheniformis

A technology of Bacillus licheniformis and biological flocculant, which is applied in the field of preparing biological flocculants by using Bacillus licheniformis, can solve the problems of low raw material cost, industrial application potential, high raw material cost, low flocculation efficiency, etc., and achieves good promotion value and raw material cost. Low, short fermentation cycle effect

Inactive Publication Date: 2009-08-12
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the problems of high raw material cost, long fermentation period and low flocculation efficiency in the current production of microbial flocculants, and provide a kind of lichen spore-utilizing agent with high flocculation activity, low raw material cost and great potential for industrial application. Method for preparing bioflocculant by bacillus

Method used

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  • Method for preparing bioflocculation by Bacillus licheniformis
  • Method for preparing bioflocculation by Bacillus licheniformis
  • Method for preparing bioflocculation by Bacillus licheniformis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Fermentation process curve of Bacillus licheniformis synthetic bioflocculant

[0047] The lawn on the fresh slope was transferred to 100 mL seed culture medium, and cultured on a shaker at 37°C at a rotation speed of 195 r / min. After 20 hours, the lawn was transferred to the fermentation medium at a 4% inoculum amount, and the cultivation was continued under the same conditions. Determine the flocculation activity of the fermentation broth at different times, the results are as follows figure 1 Shown. From figure 1 It can be seen that the flocculation activity reached 716U / mL at 44h of fermentation.

[0048] Seed medium (g / L): glucose 10, yeast extract 0.5, urea 0.5, KH 2 PO 4 0.1, NaCl 0.1, MgSO 4 ·7H 2 O0.2, distilled water, pH 7.2.

[0049] Fermentation medium (g / L): molasses 10, yeast extract 1, urea 1, KH 2 PO 4 0.1, K 2 HPO 4 0.1, NaCl 0.1, MgSO 4 ·7H 2 O 0.2, distilled water, pH 7.2.

Embodiment 2

[0050] Example 2 Ultraviolet scanning spectrum of biological flocculant

[0051] Centrifuge 50 mL of fermentation broth at 4500 r / min for 20 min to remove the precipitate and collect the supernatant. Add 2 times the volume of 95% ethanol to the supernatant. After standing overnight at 4°C, centrifuge again at 4500r / min for 20min; repeat the above operation twice. Add 1 volume of hexadecyltrimethylammonium bromide with a concentration of 2% to the ethanol precipitate, centrifuge at 4500 r / min, remove the supernatant to collect the precipitate. Dissolve the precipitate in 4% NaCl solution and stir for 2 hours, add 2 times the volume of absolute ethanol, stir well, let stand overnight at 4°C, centrifuge to remove the supernatant to obtain the precipitate, freeze the precipitate and vacuum dry to obtain the pure product Biological flocculant. The purified biological flocculant is diluted with water and scanned by UV-Vis spectrophotometry, the results are as follows figure 2 Shown. Fr...

Embodiment 3

[0052] Example 3 Infrared spectrum of biological flocculant

[0053] The purified biological flocculant in Example 2 was scanned by a Fourier transform infrared spectrometer, and the results were as follows image 3 Shown. From image 3 It can be seen that the biological flocculant is at 2927.96cm -1 Absorption, the result of C-H asymmetric stretching vibration, this absorption peak is the characteristic peak of sugars; 3423.68cm -1 There is a strong and broad absorption peak, which is caused by the vibration of -OH and N-H in the sugar ring, indicating that there are hydrogen bonds in the molecule; 1637.14cm -1 The absorption peak is the acetylamino (-NHCOCH 3 ) Caused by the C=O bond stretching vibration; 1384.28cm -1 The absorption peak is -COO in the carboxyl group - The embodiment of the symmetrical stretching vibration of the C=O bond; 1003.48cm -1 The strong absorption peak is the C-O-C antisymmetric stretching vibration absorption band in the ester. The infrared spectrum not...

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Abstract

The invention provides a method for preparing biological flocculant by utilizing Bacillus licheniformis, which relates to a biological flocculant. The invention provides a method for preparing biological flocculant by utilizing Bacillus licheniformis, which has high flocculation activity, low cost for raw materials and great industrial application potential. A microorganism is Bacillus licheniformis. The method comprises the following steps that: lawn on a fresh inclined plane is transferred to a seed culture medium for culture and then transferred to a fermentation culture medium for culture so as to obtain biological-flocculant fermentation broth; the biological-flocculant fermentation broth is centrifuged to remove precipitate and collect supernatant; the supernatant is added with ethanol, kept to stand, centrifuged and then removed; the precipitate is added with absolute ethanol, stirred and centrifuged to remove the supernatant; ethanol precipitate is added with hexadecyl trimethyl ammonium bromide and centrifuged to remove the supernatant so as to obtain the precipitate; the precipitate is dissolved in a NaCl solution, added with the absolute ethanol, stirred, stood and centrifuged to remove the supernatant so as to obtain the precipitate; and the precipitate is frozen and dried in vacuum so as to obtain pure biological flocculant.

Description

Technical field [0001] The invention relates to a biological flocculant, in particular to a method for preparing a biological flocculant by using Bacillus licheniformis. Background technique [0002] Bioflocculant is a special polymer metabolite produced by microorganisms that can make solid suspended particles, bacterial cells and colloidal particles that are not easily degradable in liquid agglutinate and precipitate. It mainly contains protein, mucopolysaccharide, cellulose and DNA, etc. Polymer compounds. Biological flocculant is a new, efficient and cheap water treatment agent obtained through microbial fermentation, separation and extraction using biological technology (Chen Yuancai, Xiao Jin. Research and Application of Natural Organic Polymer Flocculant, Industrial Water Treatment, 1999, 19(4): 11-13). Compared with traditional inorganic and organic synthetic polymer flocculants, biological flocculants have many unique properties and advantages: ①non-toxic and harmless, h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C02F1/52C12R1/10
Inventor 何宁熊裕焱李清彪于怡卢英华王远鹏
Owner XIAMEN UNIV
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