Polysulfone dialysis membrane as well as preparation method and application thereof

A technology of polysulfone dialysis membrane and sulfone dialysis membrane, which is applied in the field of polymer medical material preparation, can solve the problems of structure and performance degradation of chloromethyl polyethersulfone membrane, increase the inactivation and denaturation of biologically active macromolecules, and achieve good The effect of anticoagulant function, meeting application requirements, and wide application prospects

Inactive Publication Date: 2017-09-19
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the amino acid modified polyethersulfone hemodialysis membrane prepared by this method has better performance stability than the membrane prepared by Chinese patent CN1680010A, the reaction of amino acid grafting onto the chloromethyl polyethersulfone membrane needs to be carried out under strong alkaline conditions. Completion under these conditions will lead to the deterioration of the structure and performance of chloromethyl polyethersulfone membrane
At the same time, for some bioactive macromolecules, this grafting method also increases the risk of inactivation and denaturation of bioactive macromolecules

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Soak the polysulfone membrane with a molecular weight cut-off of 100,000 in a Tris buffer solution with a pH value of 8.5 and a dopamine concentration of 2 mg / mL for 1 hour at 25°C, then take it out and rinse it with deionized water for 12 hours to remove excess dopamine buffer to obtain polydopamine-modified polysulfone membranes. A bovine serum albumin (BSA) buffer solution with a concentration of 1 mg / mL was filtered through the dead end of the obtained polydopamine-modified polysulfone membrane for 1 hour. After the filtration, the modified membrane was shaken and washed with PBS buffer for 30 min, and then washed three times with deionized water to remove unfixed proteins. Then rinse the membrane surface with sucrose solution to prevent the denaturation or inactivation of the protein immobilized on the membrane surface in the subsequent drying or storage process; after the final membrane is vacuum dried at normal temperature and pressure or normal temperature for 2...

Embodiment 2

[0040] Soak the polysulfone membrane with a molecular weight cut-off of 100,000 in a Tris buffer solution with a pH value of 8 and a dopamine concentration of 2 mg / mL for 3 hours at 25°C, then take it out and rinse it with deionized water for 6 hours to remove excess dopamine buffer to obtain polydopamine-modified polysulfone membranes. The dead end of the obtained polydopamine-modified polysulfone membrane was used to filter the nattokinase buffer solution with a concentration of 0.5 mg / mL for 2 hours. After the filtration, the modified membrane was shaken and washed with PBS buffer for 20 minutes, and then washed twice with deionized water to remove unfixed proteins. Then rinse the membrane surface with sucrose solution to prevent the denaturation or inactivation of the protein immobilized on the membrane surface in the subsequent drying or storage process; after the final membrane is dried under normal temperature and pressure or normal temperature and vacuum for 12 hours, ...

Embodiment 3

[0043] Soak the polysulfone membrane with a molecular weight cut-off of 50,000 in a Tris buffer solution with a pH value of 9 and a dopamine concentration of 0.5 mg / mL at 25°C for 1 hour, then take it out, rinse it with deionized water for 4 hours, and remove excess dopamine buffer to obtain polydopamine-modified polysulfone membranes. The dead end of the obtained polydopamine-modified polysulfone membrane was used to filter the lumbrokinase buffer solution with a concentration of 2 mg / mL for 0.5 hours. After the filtration, the modified membrane was shaken and washed with PBS buffer for 60 min, and then washed twice with deionized water to remove unfixed proteins. Then rinse the membrane surface with sucrose solution to prevent the denaturation or inactivation of the protein immobilized on the membrane surface in the subsequent drying or storage process; after the final membrane is vacuum dried at normal temperature and pressure or normal temperature for 16 hours, the anticoa...

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Abstract

The invention provides a polysulfone dialysis membrane as well as a preparation method and application thereof. The polysulfone dialysis membrane is formed by connecting a bioactive molecule onto a polydopamine surface modified polysulfone membrane, wherein the bioactive molecule is any one or a combination of at least two of human serum albumin, bovine serum albumin, natto kinase, lumbrokinase, thrombomodulin and hirudin. The polysulfone dialysis membrane provided by the invention can guarantee the activity of the bioactive molecule and has a good anticoagulation function; the pure water flux is 65L / m<2>.h.bar to 140L / m<2>.h.bar, the retention rate on BSA (Bull Serum Albumin) is 95 percent or above and the retention rate on lysozyme is 25 percent to 35 percent; compared with an unmodified polysulfone membrane, the adsorption amount on the BSA, fibrinogen and blood platelets is remarkably reduced. The method provided by the invention has moderate conditions and a simple preparation process; the deterioration of membrane structure and performance and the inactivation or denaturation of bioactive macromolecules are not caused; the polysulfone dialysis membrane has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of polymer medical material preparation, and relates to a polysulfone dialysis membrane and its preparation method and application. Background technique [0002] Coagulation and thrombus formation are the biggest challenges during hemodialysis. The initial reaction of blood in contact with exogenous membrane surfaces is rapid adsorption of plasma proteins, followed by platelet adhesion and activation, and finally thrombus formation. Key factors in thrombus formation depend on the physicochemical properties of the membrane surface and the interaction of the membrane surface with plasma proteins and platelets. In order to solve this problem, many materials have been used to improve the anticoagulant performance of hemodialysis membranes by means of blending, surface coating, and surface grafting. Commonly used modified materials include: hydrophilic polymers (such as polyethylene glycol, polyvinylpyrrolidone), heparin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D71/68B01D69/02B01D67/00
Inventor 陈向荣万印华吉明波罗建泉杭晓风
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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